Gene expression and functional analysis of zebrafish larval fin fold regeneration
Gene expression and functional analysis of zebrafish larval fin fold regeneration
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DOI：
10.1016/j.ydbio.2008.09.028
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Kawakami, Atsushi
中科院分区：
文献类型：
--
作者：
Yoshinari, Nozomi;Ishida, Takashi;Kawakami, Atsushi
与包括人类在内的许多高等脊椎动物相比，硬骨鱼具有非凡的再生身体部位的能力。为了促进分子和遗传方法的再生，我们以前建立了使用斑马鱼幼体鳍折叠的AIL分析。在这里，我们进行了转录图谱分析，并确定了再生过程中差异控制的基因。从上调的转录本中，我们确定了一些具有局部表达的基因。令人惊讶的是，所有已鉴定的基因也在再生的成鳍中被诱导，其组织来源与幼体鳍折不同。这一结果支持与组织类型和分期无关的再生的共性。重要的是，我们的分析表明，再生组织具有比通常假设的更多的隔室，即胚泡和伤口表皮。通过药理学和遗传学方法，我们进一步评估了诱导分子的功能参与。抑制MMP9功能会损害正常的形态恢复，而不会干扰细胞的增殖。胚泡基因hspa9和SMARCA4的基因突变通过损害胚泡细胞的增殖来破坏鳍折的再生。因此，我们的结果表明，斑马鱼幼鱼的再生模型为剖析再生过程提供了一个强有力的实验手段。(C)2008 Elsevier Inc.保留所有权利。
Teleost fish have a remarkable ability to regenerate their body parts compared to many higher vertebrates including humans. To facilitate molecular and genetic approaches for regeneration, we previously established ail assay using the fin fold of zebrafish larvae. Here, we performed transcriptional profiling and identified genes differentially controlled during regeneration. From up-regulated transcripts, we identified a number of genes with localized expressions. Strikingly, all identified genes were also induced in the regenerating adult fin, which has a different tissue origin from the larval fin fold. This result supports the commonality of regeneration irrespective of tissue type and stage. Importantly, our analysis suggested that the regenerating tissue had many more compartments than generally assumed ones, the blastema and wound epidermis. By pharmacological and genetic approaches, we further evaluated functional involvement of induced molecules. Inhibition of Mmp9 function impaired proper morphological restoration without disturbing cell proliferation. Genetic mutations of blastema genes, hspa9 and smarca4, disrupted the fin fold regeneration by impairing the blastema cell proliferation. Thus, our results demonstrate that the regeneration model of juvenile zebrafish offers a powerful assay to dissect the regeneration processes. (C) 2008 Elsevier Inc. All rights reserved.