Molecular identification and characterization of the pyruvate decarboxylase gene family associated with latex regeneration and stress response in rubber tree
Molecular identification and characterization of the pyruvate decarboxylase gene family associated with latex regeneration and stress response in rubber tree
复制标题
橡胶树乳胶再生和胁迫反应相关丙酮酸脱羧酶基因家族的分子鉴定和表征
DOI：
10.1016/j.plaphy.2014.12.005
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发表时间：
2015-02-01
影响因子：
6.5
通讯作者：
Tang, Chaorong
中科院分区：
文献类型：
--
作者：
Long, Xiangyu;He, Bin;Tang, Chaorong
在植物中，乙醇发酵不仅发生在厌氧条件下，也发生在好氧条件下，并涉及碳水化合物和能量代谢。丙酮酸脱羧酶（Pyruvate decarboxylase， PDC）是乙醇发酵的第一酶和关键酶，是丙酮酸糖酵解途径的分支。本文从橡胶树中分离鉴定了4个PDC基因，它们编码的蛋白序列非常相似。在原生橡胶树中，HbPDC4的表达模式与采胶模拟橡胶产量密切相关，表明它在乳胶再生过程中调节糖代谢中发挥重要作用。HbPDC1、HbPDC2和HbPDC3在橡胶树叶片和树皮中对干旱、低温和高温胁迫有显著的表达响应，表明HbPDC1基因参与橡胶树生长发育过程中应对各种非生物和生物胁迫的自我保护和防御。为了了解橡胶树中的乙醇发酵，未来有必要对控制HbPDCs的调控途径进行深入研究。(C) 2014 Elsevier Masson SAS。版权所有。
In plants, ethanolic fermentation occurs not only under anaerobic conditions but also under aerobic conditions, and involves carbohydrate and energy metabolism. Pyruvate decarboxylase (PDC) is the first and the key enzyme of ethanolic fermentation, which branches off the main glycolytic pathway at pyruvate. Here, four PDC genes were isolated and identified in a rubber tree, and the protein sequences they encode are very similar. The expression patterns of HbPDC4 correlated well with tapping-simulated rubber productivity in virgin rubber trees, indicating it plays an important role in regulating glycometabolism during latex regeneration. HbPDC1, HbPDC2 and HbPDC3 had striking expressional responses in leaves and bark to drought, low temperature and high temperature stresses, indicating that the HbPDC genes are involve in self-protection and defense in response to various abiotic and biotic stresses during rubber tree growth and development. To understand ethanolic fermentation in rubber trees, it will be necessary to perform an in-depth study of the regulatory pathways controlling the HbPDCs in the future. (C) 2014 Elsevier Masson SAS. All rights reserved.