Studying RNA-RNA and RNA-protein interactions by isothermal titration calorimetry.
Studying RNA-RNA and RNA-protein interactions by isothermal titration calorimetry.
复制标题
DOI：
10.1016/s0076-6879(09)68019-8
复制
发表时间：
2009
影响因子：
--
通讯作者：
Feig, Andrew L.
中科院分区：
文献类型：
--
作者：
Feig, Andrew L.
等温滴定量热法（ITC）提供了一种灵敏而准确的方法，用于研究RNA折叠，RNA与小分子结合以及RNA-蛋白质相互作用的热力学。极端敏感仪器的出现和ITC在共享设施中的日益可用性使其作为RNA生物化学的工具越来越有价值。作为一种等温测量，它允许在定义的温度下进行分析，将其与提供特定于熔融温度的热力学信息的热熔融方法（例如UV熔融和差示扫描量热法）区分开来。残余结构在低温下在未折叠状态和热容量的变化导致ITC测量的热力学值和那些来自熔化研究之间的潜在差异。本文介绍了ITC如何应用于RNA生物化学的研究。
Isothermal Titration Calorimetry (ITC) provides a sensitive and accurate means by which to study the thermodynamics of RNA folding, RNA binding to small molecules, and RNA–protein interactions. The advent of extremely sensitive instrumentation and the increasing availability of ITC in shared facilities have made it increasingly valuable as a tool for RNA biochemistry. As an isothermal measurement, it allows analysis at a defined temperature, distinguishing it from thermal melting approaches (UV melting and differential scanning calorimetry, for instance) that provide thermodynamic information specific to the melting temperature. Residual structures at low temperature in the unfolded state and heat capacity changes lead to potential differences between thermodynamic values measured by ITC and those derived from melting studies. This article describes how ITC can be put to use in the study of RNA biochemistry.