Highly Hydrophilic, Two-photon Fluorescent Terpyridine Derivatives Containing Quaternary Ammonium for Specific Recognizing Ribosome RNA in Living Cells
Highly Hydrophilic, Two-photon Fluorescent Terpyridine Derivatives Containing Quaternary Ammonium for Specific Recognizing Ribosome RNA in Living Cells
复制标题
含有季铵的高亲水性双光子荧光三联吡啶衍生物，用于特异性识别活细胞中的核糖体 RNA
DOI：
10.1021/acsami.7b08068
复制
发表时间：
2017-09-20
影响因子：
9.5
通讯作者：
Tian, Yupeng
中科院分区：
文献类型：
--
作者：
Du, Wei;Wang, Hui;Tian, Yupeng
从三种带有季铵基团的水溶性三联吡啶衍生物(J1-J3)中筛选出一种选择性染色细胞内核仁RNA的双光子荧光探针(J1)。从实验和理论上系统地研究了J1-J3的光物理性质，揭示了J1-J3具有大的斯托克斯位移，并且在近红外区域双光子吸收作用截面范围为38至97 GM。这表明，体外识别实验、双光子成像实验和体内受激发射损耗实验表明，J1可以通过靶向核仁rRNA来特异性染色核仁。通过实验和分子建模研究了 JI 与核仁 rRNA 结合的作用机制。相对于胞质 RNA，J1 对核仁 RNA 的高结合选择性使得该探针成为活细胞中 rRNA 探针可视化的潜在候选者。
A two-photon fluorescent probe (J1) that selectively stains intracellular nucleolar RNA was screened from three water-soluble terpyridine derivatives (J1-J3) with quaternary ammonium groups. The photophysical properties of J1-J3 were systemically investigated both experimentally and theoretically, revealing that J1-J3 possess large Stokes shifts and the two-photon absorption action cross sections range from 38 to 97 GM in the near-infrared region. This indicates that J1 could specifically stain nucleoli by targeting the nucleolar rRNA from the recognition experiments in vitro, the two-photon imaging experiments, and the stimulated emission depletion in vivo. The mechanism of action in which JI binds to the nucleolar rRNA was researched via both experiments and molecular modeling. The high binding selectivity of J1 to nucleolar RNA over cytosolic RNA made this probe a potential candidate to visualize rRNA probe in the living cells.