Isolation of bacteria other than Helicobacter pylori from stomachs of squirrel monkeys (Saimiri spp.) with gastritis
Isolation of bacteria other than Helicobacter pylori from stomachs of squirrel monkeys (Saimiri spp.) with gastritis
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DOI：
10.1023/a:1005496022881
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发表时间：
2000-02-01
影响因子：
3.1
通讯作者：
Stadtländer, CTKH
中科院分区：
文献类型：
--
作者：
Khanolkar-Gaitonde, SS;Reubish, GK;Stadtländer, CTKH
取自12只松鼠猴(Saimiri spp.)通过培养来调查细菌的存在。用VITEK和API NFT方法(BioMerieux)对两只患胃炎的猕猴胃部定植革兰氏阴性、尿素酶阳性细菌，鉴定为人Ochrobactrum Peopi。第三只患有胃炎的猴子对沙门氏气单胞菌和水泡假单胞菌呈阳性(均为尿素酶阴性)。未从松鼠猴体内分离到幽门螺杆菌。光镜和透射电子显微镜检查显示，人按蚊分离株表面覆盖有胞外物质，表明有囊膜存在。结果表明，S664和S1835菌株的米氏常数(K-m)分别为6.2mM和4.0mM，模式菌株49188的米氏常数为3.7mM。用幽门螺杆菌和人幽门螺杆菌特异性抗体进行的免疫印迹分析检测到幽门螺杆菌、人幽门螺杆菌和人幽门螺杆菌的尿素酶之间有共同的抗原表位。在6%非变性凝胶上测得人按蚊分离株尿素酶的表观分子量约为82 kDa。采用DADE国际公司的MicroScan方法进行药敏试验，结果显示人间血吸虫分离株对阿米卡星、环丙沙星、庆大霉素、头孢哌酮、妥布霉素、亚胺培南和甲氧嘧啶/磺胺甲恶唑敏感。
Gastric biopsy specimens obtained from 12 squirrel monkeys (Saimiri spp.) were investigated by culture for the presence of bacteria. The stomachs of two monkeys with gastritis were colonized with gram-negative, urease-positive bacteria, identified as Ochrobactrum anthropi by the Vitek and API NFT methods (BioMerieux). A third monkey with gastritis was positive for Aeromonas salmonicida and Pseudomonas vesicularis (both urease-negative). No Helicobacter pylori was isolated from squirrel monkeys. Light microscopic and transmission electron microscopic examination revealed that the O. anthropi isolates were covered by extracellular material, indicating a capsule. Characterization of the O. anthropi urease revealed Michaelis-Menten constants (K-m values) of 6.2 and 4.0 mM urea for the ureases of O. anthropi isolates S664 and S1835, respectively, and 3.7 for type strain 49188. Western blot analysis using H. pylori- and H. felis-specific antibodies detected shared antigenic epitopes between the ureases of H. pylori, H. felis, and O. anthropi. The apparent molecular mass of the urease enzymes of the O. anthropi isolates was determined on 6% nondenaturing gels to be approximately 82 kDa. Antimicrobial susceptibility tests, using the MicroScan method (Dade International), revealed multidrug resistance for the O. anthropi isolates with susceptibilities for the antibiotics amikacin, ciprofloxacin, gentamicin, cefoperazone, tobramycin, imipenem, and trimethoprim/sulfamethoxazole.