Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy.
Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy.
复制标题
通过免疫荧光显微镜观察具核梭杆菌细胞壁水解酶抑制剂。
DOI：
10.1007/978-1-0716-3491-2_3
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Ton-That,Hung
中科院分区：
文献类型：
--
作者：
To,Kevin;Britton,Timmie;Ton-That,Hung
Innately present in tears, saliva and mucosal secretions, lysozyme provides a critical defensive strategy to the host by cleaving the β-1,4-glycosidic bonds betweenN-acetylmuramic acid andN-acetyl-D-glucosamine residues of peptidoglycan of invading bacteria, leading to bacterial lysis. To counter this class of cell wall hydrolase enzymes, bacteria produce several lysozyme inhibitors, a representative of which, MliC, was identified inEscherichia coli,Pseudomonas aeruginosa, and various bacterial species. The Gram-negative oral anaerobeFusobacterium nucleatumencodes an uncharacterized lipoprotein homologous to MliC, whose localization is unknown. Here, we provide an experimental procedure to localize this MliC-like lipoprotein by employing immunofluorescence microscopy. In principle, this protocol can be used for any bacterial system to monitor protein localization.
