Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis
Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis
复制标题
通过过表达树干树干木糖醇脱氢酶基因的解脂耶氏酵母工程菌株高效生产 D-苏糖醇
DOI：
10.1016/j.bej.2019.107259
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发表时间：
2019-09-15
影响因子：
3.9
通讯作者：
Cheng, Hairong
中科院分区：
文献类型：
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作者：
Chi, Ping;Wang, Siqi;Cheng, Hairong
d -苏糖醇（以下简称苏糖醇）是赤藓糖醇的非对映异构体，在绿色化学、食品、制药、医药等领域有着广泛的应用。本研究证明，来自Scheffersomyces stipitis的木糖醇脱氢酶CBS 6054能够以赤藓糖糖为中间体，高效催化赤藓糖醇生成苏糖醇。当相应基因Ss-XDH在产赤糖醇的多脂耶氏菌CGMCC7326中过表达时，产赤糖醇滴度为112 g。L-1，葡萄糖产率0.37。出乎意料的是，编码甘露醇脱氢酶的基因在苏糖醇产生染色中被上调，导致甘露醇在培养液中积累。为了消除这一副产物和共同生产赤藓糖醇，开发了基于假丝酵母培养的第二工艺步骤，以促进后续的苏糖醇纯化。本文首次报道了利用工程解脂酵母和野生型假丝酵母菌从葡萄糖中有效地合成苏糖醇。
D-threitol (hereinafter as threitol) is a diastereoisomer of erythritol with wider applications in green chemistry, food, pharmaceutical, and medicine. Herein, xylitol dehydrogenase from Scheffersomyces stipitis CBS 6054 was demonstrated able to catalyze highly efficient threitol formation from erythritol with erythrulose as an intermediate. When the corresponding gene Ss-XDH was overexpressed in the erythritol-producing Yarrowia lipolytica strain CGMCC7326, threitol was produced with a titer of 112 g.L-1 and yield of 0.37 from glucose. Unexpectedly, gene encoding mannitol dehydrogenase was upregulated in the threitol producing stain, leading to mannitol accumulation in the culture broth. In order to eliminate this byproduct and coproduced erythritol, a second process step based on the culture of Candida parapsilosis was developed to facilitate subsequent threitol purification. This is the first report to efficiently biosynthesize threitol from glucose through the fermentation process by an engineered Y. lipolytica and wild-type Candida parapsilosis.