Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.
Thermoflexus hugenholtzii gen. nov., sp nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov and Thermoflexales ord. nov.
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DOI：
10.1099/ijs.0.055855-0
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发表时间：
2014-06-01
影响因子：
2.8
通讯作者：
Hedlund, Brian P.
中科院分区：
文献类型：
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作者：
Dodsworth, Jeremy A.;Gevorkian, Jonathan;Hedlund, Brian P.
从美国内华达州大沸腾泉的沉积物中分离出一种嗜热、丝状、异养细菌，称为菌株 JAD2(T)，它是一种尚未培养的谱系的成员，该谱系存在于世界各地的一些温泉中，有时甚至含量丰富。细胞的平均直径为0.3μm，长度为4.0μm，并形成长度通常为20至200μm的细丝。丝状革兰氏染色反应呈阴性，未形成孢子且未观察到运动性。最适生长温度为72.5-75℃，范围为67.5-75℃；最适生长pH为6.75，范围为6.5-7.75。当补充维生素时，蛋白胨、胰蛋白胨或酵母提取物能够支持生长，但使用各种确定的有机底物时没有观察到生长。菌株 JAD2(T) 是微需氧和兼性厌氧的，最佳生长条件为 1% (v/v) O-2，上限为 8% O-2。主要细胞脂肪酸 (>5%) 为 C-16:0、C-19:0、C-18:0、C-20:0 和 C-19:1。基因组DNA G+C含量为69.3mol%。使用 16S rRNA 基因和其他保守基因的序列进行系统发育和系统基因组分析，将 JAD2(T) 置于 Chloroflexi 门内，但不属于该门中任何现有的类别。这些结果表明菌株 JAD2(T) 是 Chloroflexi 门内新谱系的第一个培养代表，我们建议将其命名为 Thermoflexus Hugenholtzii gen。十一月，SP。 11 月，在 Thermoflexia classis 11 内，Thermoflexales 秩序。十一月和Thermoflexaceae家族。十一月Thermoflexus Hugenholtzii 的模式菌株是 JAD2(T) (=JCM 19131(T)=CCTCC AB-2014030(T))。
A thermophilic, filamentous, heterotrophic bacterium, designated strain JAD2(T), a member of an as-yet uncultivated lineage that is present and sometimes abundant in some hot springs worldwide, was isolated from sediment of Great Boiling Spring in Nevada, USA. Cells had a mean diameter of 0.3 mu m and length of 4.0 mu m, and formed filaments that typically ranged in length from 20 to 200 mu m. Filaments were negative for the Gram stain reaction, spores were not formed and motility was not observed. The optimum temperature for growth was 72.5-75 degrees C, with a range of 67.5-75 degrees C, and the optimum pH for growth was 6.75, with a range of pH 6.5-7.75. Peptone, tryptone or yeast extract were able to support growth when supplemented with vitamins, but no growth was observed using a variety of defined organic substrates. Strain JAD2(T) was microaerophilic and facultatively anaerobic, with optimal growth at 1% (v/v) O-2 and an upper limit of 8% O-2. The major cellular fatty acids (>5%) were C-16:0, C-19:0, C-18:0, C-20:0 and C-19:1. The genomic DNA G+C content was 69.3 mol%. Phylogenetic and phylogenomic analyses using sequences of the 16S rRNA gene and other conserved genes placed JAD2(T) within the phylum Chloroflexi, but not within any existing class in this phylum. These results indicate that strain JAD2(T) is the first cultivated representative of a novel lineage within the phylum Chloroflexi, for which we propose the name Thermoflexus hugenholtzii gen. nov., sp. nov., within Thermoflexia classis nov., Thermoflexales ord. nov. and Thermoflexaceae fam. nov. The type strain of Thermoflexus hugenholtzii is JAD2(T) (=JCM 19131(T)=CCTCC AB-2014030(T)).