Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
复制标题
酵母全细胞提取物中 RNA 聚合酶 I 的特异性引发。
DOI：
10.1073/pnas.88.3.1004
复制
发表时间：
1991
影响因子：
11.1
通讯作者：
Reeder,RH
中科院分区：
文献类型：
--
作者：
Schultz,MC;Choe,SY;Reeder,RH
描述了一种从酵母(酿酒酵母)中制备可溶性全细胞提取物的方法，该提取物支持RNA聚合酶I、II和III的活性和特异性转录启动。在高密度培养中，聚合酶I的特异性启动减少，与内源35S rRNA前体丰度的减少平行。这种提取物对于研究酵母中调节rRNA转录的分子机制是有用的。
A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.