Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.
Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.
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3:5-环核苷酸磷酸二酯酶和肌动球蛋白 ATP 酶的 Ca2 依赖性调节蛋白之间的结构相似性。
DOI：
10.1016/s0021-9258(17)33231-3
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
T. Vanaman
中科院分区：
文献类型：
--
作者：
D. M. Watterson;W. Harrelson;P. Keller;F. Sharief;T. Vanaman
本文报道了从牛脑中分离出的3 '：5'-环核苷酸磷酸二酯酶的钙依赖性蛋白调节剂的研究结果，结果表明它与肌钙蛋白的钙结合亚基具有结构相似性。这两种蛋白质具有封闭的NH 2末端，相似和特征的紫外吸收光谱，相似的Ca 2+结合特性，非常相似的氨基酸组成，并在十二烷基硫酸钠-聚丙烯酰胺凝胶上共迁移。从牛脑调节蛋白中分离的所选胰蛋白酶肽的一级结构与兔骨骼肌和牛心肌肌钙蛋白C的一级序列的区域相似或相同。牛脑调节蛋白含有肌钙蛋白C中未发现的茚三酮阳性碱性化合物。提出了一种改进的方法，每公斤牛脑可产生40至70毫克的调节蛋白。
Results of studies of the Ca2+-dependent protein modulator of 3':5'-cyclic nucleotide phosphodiesterase isolated from bovine brain are presented which show its structural similarity to the Ca2+-binding subunit of muscle troponin. Both proteins have blocked NH2 termini, similar and characteristic ultraviolet absorption spectra, similar Ca2+-binding properties, very similar amino acid compositions, and co-migrate on sodium dodecyl sulfate-polyacrylamide gels. The primary structures of selected tryptic peptides isolated from bovine brain modulator protein are similar or identical with regions of the primary sequences of rabbit skeletal muscle and bovine cardiac muscle troponin C. Bovine brain modulator protein contains and unidentified ninhydrin-positive basic compound not found in muscle troponin C. An improved procedure is presented which yields 40 to 70 mg of modulator protein per kg of bovine brain.