Influenza virus serology - a comparative study
Influenza virus serology - a comparative study
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DOI：
10.1016/s0166-0934(98)00174-8
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发表时间：
1999-03-01
影响因子：
3.1
通讯作者：
Osterhaus, ADME
中科院分区：
文献类型：
--
作者：
Rothbarth, PH;Groen, J;Osterhaus, ADME
病毒分离或流感病毒抗原检测是流感病毒感染急性期最快速的诊断方法。由于血清学更容易进行，因此在两个明确定义的患者组中研究了血清IgM、伊加和IgG的合成，这两个患者组感染了B型流感病毒（队列1，n = 37）和A型流感病毒（队列2，n = 40），在症状发作后36小时内通过抗原检测和/或病毒分离进行诊断。在13例（35%）流感B患者中发现IgM，12例（32%）流感患者中发现伊加，而在33例（92%）中通过酶联免疫吸附试验（ELISA）和74%通过血凝抑制试验（HAI）发现抗体显著升高。对于甲型流感队列，这些数字分别为18（45%）、27（68%）和24（62%）例HAI（72%）。在年龄匹配的对照组中，在入组患者发病第一天流血的，发现伊加和IgG的患病率较低，B型流感的患病率分别为2%和18%，甲型流感的患病率分别为4%和39%。研究抗体应答的动力学，我们发现病毒特异性伊加和大量的IgE在感染的第一周内合成。它的结论是，一个特定的血清伊加的发现是高度指示急性流感感染。(C)1999 Elsevier Science B. V.保留所有权利。
Virus isolation or influenza virus antigen detection are the most rapid tests for diagnosis in the acute stage of influenza virus infection. As serology is easier to carry out, the synthesis of serum IgM, IgA and IgG was studied in two well-defined patient groups, infected with influenza B virus (cohort 1, n = 37) and influenza A virus (cohort 2, n = 40), diagnosed by antigen detection and/or Virus isolation within 36 h after onset of symptoms. IgM was found in 13 influenza B patients (35%), IgA in 12 patients (32%), whereas a significant antibody rise was found in 33 patients (92%) by enzyme-linked immunosorbent assay (ELISA) and 74% by haemagglutination inhibition assay (HAI). For the influenza A cohort these numbers were respectively 18 (45%), 27 (68%) and 24 (62%) HAI (72%). In age-matched controls, who were bled on the first day of illness of the enrolled patient low prevalence was found for IgA and IgG, for influenza B respectively in 2 and 18%, and for influenza A in 4 and 39%. Studying the kinetics of the antibody response, we found that virus specific IgA and the bulk of Ige is synthesised within the first week of the infection. It is concluded that the finding of a specific serum IgA is highly indicative of an acute influenza infection. (C) 1999 Elsevier Science B.V. All rights reserved.