CONSTITUTIVE EXPRESSION OF IRON-ENTEROCHELIN AND FERRICHROME UPTAKE SYSTEMS IN A MUTANT STRAIN OF SALMONELLA-TYPHIMURIUM
CONSTITUTIVE EXPRESSION OF IRON-ENTEROCHELIN AND FERRICHROME UPTAKE SYSTEMS IN A MUTANT STRAIN OF SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.135.3.928-934.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
ROTHFIELD, LI
中科院分区：
文献类型：
--
作者：
ERNST, JF;BENNETT, RL;ROTHFIELD, LI
已知S.鼠伤寒沙门氏菌，一种利用铁-肠螯合素，另一种利用铁色素。先前表明，Fe-肠螯合素摄取系统的几个元素的表达受培养基的Fe含量的调节，在高铁培养基中生长导致肠螯合素合成和降解的酶的抑制以及整个细胞摄取Fe-肠螯合素的能力。突变株在高铁培养基中的生长与组成型表达：铁-肠螯合素摄取的全细胞;铁色素摄取的全细胞;肠螯合素的合成;铁-肠螯合素的细胞内降解;和3个主要的外膜蛋白（OM 1，OM 2和OM 3）的合成，进行了描述。在野生型菌株中，这些特性仅在铁缺乏培养基中生长后表达。该突变影响负责调节高亲和力铁吸收系统组分的结构基因表达的基因。术语毛皮，铁吸收调节，建议这类新的突变体。
Two high-affinity Fe uptake systems are known in S. typhimurium, one utilizing Fe-enterochelin and the other utilizing ferrichrome. It was shown previously that expression of several elements of the Fe-enterochelin uptake system are regulated by the Fe content of the medium, with growth in high-Fe medium resulting in repression of enzymes of enterochelin synthesis and degradation and of the ability of whole cells to take up Fe-enterochelin. A mutant strain in which growth in high-Fe medium was associated with constitutive expression of: Fe-enterochelin uptake by whole cells; ferrichrome uptake by whole cells; synthesis of enterochelin; intracellular degradation of Fe-enterochelin; and synthesis of 3 major outer-membrane proteins (OM1, OM2 and OM3), was described. In the wild-type strain these properties were expressed only after growth in Fe-deficient medium. The mutation affects a gene responsible for regulating expression of the structural genes for the components of the high-affinity Fe uptake systems. The term fur, for Fe uptake regulation, is suggested for this new class of mutant.