High-precision, non-invasive anti-microvascular approach via concurrent ultrasound and laser irradiation.
High-precision, non-invasive anti-microvascular approach via concurrent ultrasound and laser irradiation.
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DOI：
10.1038/srep40243
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发表时间：
2017-01-11
影响因子：
4.6
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Hu Z;Zhang H;Mordovanakis A;Paulus YM;Liu Q;Wang X;Yang X
抗血管治疗代表了一种经证实的治疗血管生成的策略。通过应用同步超声脉冲和纳秒激光照射，我们开发了一种新的，选择性的，非侵入性的，局部抗血管的方法，称为光介导的超声治疗（PUT）。PUT利用生物组织之间的高天然光学对比度，可以治疗微血管，而不会对周围组织造成附带损伤。在鸡卵黄囊膜模型中，在相同的超声参数（1 MHz，0.45 MPa，10 Hz，占空比10%）下，4 mJ/cm 2和6 mJ/cm 2激光能量密度的PUT分别诱导51%（p = 0.001）和37%（p = 0.018）的血管直径减小。在8 mJ/cm ~ 2激光能量密度下，PUT可使血管破裂率达90%，P < 0.01。通过利用578 nm或650 nm的激光波长证明了PUT的选择性，其中PUT选择性地收缩静脉或闭塞的动脉。在兔耳模型中，PUT在7天后诱导血液灌注减少68.5%（p < 0.001），而不损伤周围细胞。在人体血液中的体外实验表明，空化可能在PUT中发挥作用。总之，PUT作为一种新型的非侵入性抗血管方法，具有精确靶向血管的能力，具有重要的前景。
Antivascular therapy represents a proven strategy to treat angiogenesis. By applying synchronized ultrasound bursts and nanosecond laser irradiation, we developed a novel, selective, non-invasive, localized antivascular method, termed photo-mediated ultrasound therapy (PUT). PUT takes advantage of the high native optical contrast among biological tissues and can treat microvessels without causing collateral damage to the surrounding tissue. In a chicken yolk sac membrane model, under the same ultrasound parameters (1 MHz at 0.45 MPa and 10 Hz with 10% duty cycle), PUT with 4 mJ/cm2 and 6 mJ/cm2 laser fluence induced 51% (p = 0.001) and 37% (p = 0.018) vessel diameter reductions respectively. With 8 mJ/cm2 laser fluence, PUT would yield vessel disruption (90%, p < 0.01). Selectivity of PUT was demonstrated by utilizing laser wavelengths at 578 nm or 650 nm, where PUT selectively shrank veins or occluded arteries. In a rabbit ear model, PUT induced a 68.5% reduction in blood perfusion after 7 days (p < 0.001) without damaging the surrounding cells. In vitro experiments in human blood suggested that cavitation may play a role in PUT. In conclusion, PUT holds significant promise as a novel non-invasive antivascular method with the capability to precisely target blood vessels.