Increased nerve growth factor levels in spontaneously hypertensive rats
Increased nerve growth factor levels in spontaneously hypertensive rats
复制标题
自发性高血压大鼠神经生长因子水平升高
DOI：
10.1097/00004872-199203000-00003
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发表时间：
1992
影响因子：
4.9
通讯作者：
S. Furukawa
中科院分区：
文献类型：
--
作者：
T. Ueyama;M. Hamada;T. Hano;I. Nishio;Y. Masuyama;S. Furukawa
目的：在自发性高血压大鼠（SHR）中已经报道了交感神经支配增加;然而，所涉及的确切机制尚不清楚。神经生长因子（NGF），周围交感神经元中的神经营养肽，被认为有助于这种现象。方法：测定SHR和正常WKY大鼠发育过程中神经生长因子（NGF）的含量。分离肠系膜动脉、脾、心脏和坐骨神经并匀浆。使用高灵敏度和特异性的双位点酶免疫测定法测定上清液中的NGF含量。结果：3周龄时，SHR大鼠脾脏、坐骨神经和肠系膜动脉中的NGF含量均高于WKY大鼠。然而，这些差异在12周龄时完全消失。心脏神经生长因子含量略低于3周龄的SHR，相反，12周龄的SHR高于年龄匹配的WKY大鼠。结论：这些研究结果表明，除了心脏，观察到的SHR组织在年轻时过度产生NGF，导致外周交感神经系统活动的增强和血管收缩性儿茶酚胺的产生。
Objective: Increased sympathetic innervation has been reported in spontaneously hypertensive rats (SHR); however, the precise mechanisms involved are not yet clear. Nerve growth factor (NGF), a neurotrophic peptide in peripheral sympathetic neurons, is believed to contribute to this phenomenon. Methods: We measured the content of NGF in SHR and control Wistar–Kyoto (WKY) rats during development. Mesenteric artery, spleen, heart and sciatic nerve were isolated and homogenized. NGF content in the supernatant fractions was measured using a highly sensitive and specific two-site enzyme immunoassay. Results: At 3 weeks of age, SHR had a greater NGF content in the spleen, the sciatic nerve and the mesenteric artery than WKY rats. However, these differences disappeared completely at 12 weeks of age. Cardiac NGF content was slightly lower in 3-week-old SHR and, conversely, higher in 12-week-old SHR than in age-matched WKY rats. Conclusions: These findings suggest that, except for the heart, the SHR tissues observed overproduce NGF at a young age, leading to enhancement of peripheral sympathetic nervous system activity and the production of vasoconstrictive catecholamines.