Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan
Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan
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DOI：
10.1007/s00705-016-2834-7
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发表时间：
2016-06-01
影响因子：
2.7
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
--
作者：
Naoi, Yuki;Kishimoto, Mai;Nagai, Makoto
在使用宏基因组学方法对猪粪便病毒进行调查期间，从一头两个月大的健康猪的粪便中鉴定出一种新型小核糖核酸病毒。该病毒被命名为日本猪小核糖核酸病毒（PPVJ），具有标准的小核糖核酸病毒基因组组织，包括L蛋白区。5'未翻译区含有II型内部核糖体进入位点。该病毒与P1病毒1型（氨基酸序列一致性为38.2%）、P2马鼻炎A型病毒（氨基酸序列一致性为25.8%）和P3病毒2型（氨基酸序列一致性为40.9%）亲缘关系最密切。根据小核糖核酸科的属划分（P1、P2和P3的氨基酸序列一致性分别小于40%、40%和50%），PPVJ代表了小核糖核酸科的一个新属。利用从3D序列设计的特异性引物，通过反转录PCR，在23.3%（来自广泛地区58.3%的猪场）的4个月以下猪的粪便样本中检测到PPVJ，然后进行测序。该病毒在动物中的宿主范围和致病性潜力尚未确定。
During an investigation of porcine fecal viruses using a metagenomics approach, a novel picornavirus was identified from the feces of a healthy two-month-old pig. This virus, named porcine picornavirus Japan (PPVJ), had a standard picornavirus genome organization, including the L protein region. The 5' untranslated region harbored a type II internal ribosomal entry site. This virus was most closely related to lesavirus 1 (amino acid sequence identity: 38.2 %) in P1, equine rhinitis A virus (25.8 %) in P2, and lesavirus 2 (40.9 %) in P3. According to the genus demarcations for the family Picornaviridae (less than 40 %, 40 %, and 50 % amino acid sequence identity in P1, P2, and P3, respectively), PPVJ represents a new genus in the family Picornaviridae. PPVJ was detected in 23.3 % of the fecal samples (from 58.3 % of the farms across a wide area) from pigs less than four months old, by reverse transcription PCR, using specific primers designed from the 3D sequence, followed by sequencing. The host range and pathogenic potential of this virus in animals is yet to be determined.