Identification of ubiquitin conjugated Protein Phosphatase Inhibitor 1 from postmortem bovine muscle
Identification of ubiquitin conjugated Protein Phosphatase Inhibitor 1 from postmortem bovine muscle
复制标题
死后牛肌肉中泛素结合蛋白磷酸酶抑制剂 1 的鉴定
DOI：
10.1016/j.foodchem.2009.01.061
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发表时间：
2009
期刊：
影响因子：
8.8
通讯作者：
M. Sekikawa
中科院分区：
文献类型：
--
作者：
P. Nyam;B. Jayawardana;J. A. Aro;K. Shimada;M. Fukushima;M. Sekikawa
以前，我们的特点是存在的泛素和一些泛素化的蛋白质（共轭物），这被认为是泛素共轭物在死后骨骼肌和心肌的肌浆部分使用SDS-PAGE和Western blot。在本研究中，对约27 kDa的泛素缀合物进行鉴定。通过Edman降解生成的N-末端氨基酸序列分析得到13个氨基酸的序列（KIQFTVPLLEPHL），其与蛋白磷酸酶抑制剂1的序列相似。基于该结果，通过使用蛋白磷酸酶抑制剂1的多克隆抗体进行Western印迹分析，并且缀合物被清楚地识别和反应。因此，约27 kDa的泛素化蛋白被鉴定为蛋白磷酸酶抑制剂1。
Previously, we characterised the presence of ubiquitin and some ubiquitinated proteins (conjugates), which were considered as ubiquitin conjugates in the sarcoplasmic fraction of postmortem skeletal and cardiac muscles using SDS–PAGE and Western blot. In this study, an ubiquitin conjugate around 27kDa was subjected to identification. The N-terminal amino acid sequence analysis generated by Edman degradation resulted in a 13 amino acid sequence (KIQFTVPLLEPHL), which is similar to that of Protein Phosphatase Inhibitor 1. Based on this result, Western blot analysis was performed by using a polyclonal antibody for Protein Phosphatase Inhibitor 1 and the conjugate was recognised and reacted clearly. Thus, the ubiquitinated protein around 27kDa is identified as Protein Phosphatase Inhibitor 1.
DOI：
10.1073/pnas.84.20.6970
发表时间：
1987-10-01
影响因子：
11.1
作者：
AEBERSOLD, RH;LEAVITT, J;KENT, SBH
通讯作者：
KENT, SBH