A novel mode of translocation for cytolethal distending toxin
A novel mode of translocation for cytolethal distending toxin
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DOI：
10.1016/j.bbamcr.2008.11.017
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发表时间：
2009-03-01
影响因子：
5.1
通讯作者：
Teter, Ken
中科院分区：
文献类型：
--
作者：
Guerra, Lina;Nemec, Kathleen N.;Teter, Ken
毒素催化亚基的热不稳定性可能是通过利用内质网相关降解（ERAD）机制离开内质网（ER）的毒素的共同特性。杜克雷嗜血杆菌细胞致死膨胀毒素（HdCDT）不利用ERAD离开ER，因此我们预测其催化亚基（HdCdtB）的结构特性将不同于其他ER易位毒素。在这里，我们记录了HdCdtB的热稳定特性，这将其与其他ER易位毒素区分开来。基于细胞的测定进一步表明，HdCdtB在离开ER之前不解折叠，并且它可以直接从ER腔移动到核质。这些观察结果表明HdCdtB的ER退出的新模式。(c)2008 Elsevier B.V.保留所有权利。
Thermal instability in the toxin catalytic subunit may be a common property of toxins that exit the endoplasmic reticulum (ER) by exploiting the mechanism of ER-associated degradation (ERAD). The Haemophilus ducreyi cytolethal distending toxin (HdCDT) does not utilize ERAD to exit the ER, so we predicted the structural properties of its catalytic subunit (HdCdtB) would differ from other ER-translocating toxins. Here, we document the heat-stable properties of HdCdtB which distinguish it from other ER-translocating toxins. Cell-based assays further suggested that HdCdtB does not unfold before exiting the ER and that it may move directly from the ER lumen to the nucleoplasm. These observations suggest a novel mode of ER exit for HdCdtB. (c) 2008 Elsevier B.V. All rights reserved.