A KINASE FROM FISSION YEAST RESPONSIBLE FOR BLOCKING MITOSIS IN S-PHASE
A KINASE FROM FISSION YEAST RESPONSIBLE FOR BLOCKING MITOSIS IN S-PHASE
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DOI：
10.1038/374817a0
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发表时间：
1995-04-27
期刊：
影响因子：
64.8
通讯作者：
OKAYAMA, H
中科院分区：
文献类型：
--
作者：
MURAKAMI, H;OKAYAMA, H
在几乎所有的真核生物中，有丝分裂在DNA合成完成后开始，这个有序的过程是由检查点机制确保的，该机制阻止：当DNA正在合成或被损坏时有丝分裂的开始，在裂殖酵母裂殖酵母中，这个机制涉及一些rad（+）和hus（+）基因（1-4）。然而，目前尚不清楚检查点系统如何监测这些事件，最近分离出一种温度敏感性DNA聚合酶-α突变体的多拷贝抑制基因，该基因命名为cds 1（+）。（检查DNA合成），编码一个典型的蛋白激酶，在这里，我们报告说，这种蛋白激酶是DNA复制监测S/G2检查点系统的关键组成部分，我们的数据表明，它的主要作用是通过与DNA聚合酶α相互作用来监测DNA合成，并在DNA合成进行时发送信号以阻止有丝分裂的开始。
IN virtually all eukaryotes, mitosis starts after the completion of DNA synthesis, This orderly process is ensured by the checkpoint mechanism that blocks:the onset of mitosis while DNA is being Synthesized or is damaged, In the fission yeast Schizosaccharomyces pombe, this mechanism involves some rad(+) and hus(+) genes(1-4). However, it is not known how the checkpoint system monitors these events, Recently a multicopy suppressor of a temperature-sensitive DNA polymerase-alpha mutant was isolated, This gene, named cds1(+) (checking DNA synthesis), encodes a typical protein kinase, Here we report that this protein kinase is a key component of the DNA replication-monitoring S/G2 checkpoint system, Our data suggest that its primary role is to monitor DNA synthesis by interacting: with DNA polymerase alpha and send a signal to block the onset of mitosis while DNA synthesis is in progress.