Mechanisms of active oxygen species reduction by non-steroidal anti-inflammatory drugs
Mechanisms of active oxygen species reduction by non-steroidal anti-inflammatory drugs
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DOI：
10.1016/s1357-2725(96)00144-6
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发表时间：
1997-03-01
影响因子：
4
通讯作者：
Kimura, K
中科院分区：
文献类型：
--
作者：
Kimura, K
许多形式的活性氧被认为参与了炎症过程。抗炎药被认为是活性氧抑制剂。然而，这种抑制活性的详细机制仍然不清楚，因为很少有成熟的方法来研究抗炎药物对活性氧的抑制作用。本文采用电子自旋共振自旋捕集法和辣根过氧化物酶法研究了对乙酰氨基酚、水杨酸钠、萘普生和氟比洛芬四种非甾体抗炎药对活性氧的清除和抑制能力。本实验以次黄嘌呤-黄嘌呤氧化酶反应体系中产生的超氧阴离子和自发歧化反应产生的过氧化氢为活性氧模型。这里的数据表明，超氧化物的量以反应中非甾体抗炎药浓度的方式减少。对这些反应的动力学研究搁置，对乙酰氨基酚和水杨酸钠与超氧化物竞争反应，而萘普生和氟比洛芬没有。生成分析;反应体系中超氧化物自发歧化形成的过氧化氢表明，对乙酰氨基酚会增加过氧化氢的含量，而水杨酸钠、萘普生和氟比洛芬会减少过氧化氢的含量。(C)1997 Elsevier Science Ltd.
Many forms of active oxygen have been suggested to participate in the course of inflammation. Anti-inflammatory drugs have been considered to function as active oxygen inhibitors. However, detailed mechanisms for such inhibitory activity remain unclear because of Little well established methods to study inhibitory effect of anti-inflammatory drugs on active oxygen species. In this report, the author investigated four non-steroidal anti-inflammatory drugs, including acetaminophen, sodium salicylate, naproxen and flurbiprofen, their elimination and inhibition ability of active oxygen, using of the electron spin resonance spin-trapping method and the horseradish peroxidase method. In this experiment as active oxygen models, superoxide was evolved from a hypoxanthine-xanthine oxidase reaction system, and hydrogen peroxide by the spontaneous dismutation reaction. The data here show that the amount of superoxide was reduced in the manner of concentration of non-steroidal anti-inflammatory drugs in the reaction. Kinetic studies for these reaction shelved that acetaminophen and sodium salicylate reacted with superoxide competitively, whereas naproxen and flurbiprofen did not. Analysis of generation of; hydrogen peroxide formed by the spontaneous dismutation of superoxide derived from the reaction system revealed that hydrogen peroxide was increased by acetaminophen and decreased by sodium salicylate, naproxen and flurbiprofen. (C) 1997 Elsevier Science Ltd.