Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus
Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve Unio tumidus
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DOI：
10.1016/s0166-445x(97)00024-6
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发表时间：
1997-09-01
影响因子：
4.5
通讯作者：
Vasseur, P
中科院分区：
文献类型：
--
作者：
Doyotte, A;Cossu, C;Vasseur, P
这项工作的目的是评估潜在的效用的抗氧化剂参数作为指标，暴露于有毒物质和有毒影响的淡水贻贝Unio tumidus。抗氧化酶（谷胱甘肽过氧化物酶（EC 1.11.1.9），谷胱甘肽还原酶（EC 1.6.4.2），超氧化物歧化酶（EC 1.15.1.1），过氧化氢酶（EC 1.11.1.6）），谷胱甘肽和脂质过氧化的氧化还原状态进行了测量，在暴露于铜（30 μ g l（-1））或/和福美双（100 μ g l（-1））3天后，首先在鳃和贻贝消化腺。一个复杂的工业废水对抗氧化系统的影响进行了研究后，在一个举行的研究：笼养贻贝被放置在河流上游和下游的污染源组成的焦化厂的污水1周。这些研究表明，在这两个实验暴露，最敏感的参数是硒依赖性谷胱甘肽过氧化物酶活性（SeGPx），还原型谷胱甘肽水平（GSH），特别是谷胱甘肽还原酶活性（GRd）显着下降。在铜和福美双共同暴露下，鳃中SeGPX和GRd活性的降低更为明显，分别降低了74%和78%（P < 0.01）;在这种情况下，GSH水平降低了50%。在现场研究中，活动可以减少35%和72%，分别在鳃SeGPx和GRd，还原型谷胱甘肽水平的下降可达45%。与此相反，超氧化物歧化酶和过氧化氢酶的活性保持基本恒定，在所有的治疗组与对照组相比。脂质过氧化，通过HPLC测定的丙二醛含量所表示的，在实验中与铜+福美双略有增强，这种增加（从1.8到2.8倍）是伴随着抗氧化防御的耗尽。抗氧化参数的反应总体上大于暴露贻贝的鳃消化腺。这些结果表明，在抗氧化防御系统中，SeGPx，GSH水平，特别是GRd，可以代表感兴趣的生物标志物，用于估计环境污染对淡水无脊椎动物的影响。(C)1997年Elsevier Science B.V.
The aim of this work was to evaluate the potential utility of antioxidant parameters as indicators of exposure to toxicants and of toxic effects in the freshwater mussel Unio tumidus. Antioxidant enzymes (glutathione peroxidase (EC 1.11.1.9), glutathione reductase (EC 1.6.4.2), superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6)), redox status of glutathione and lipid peroxidation were measured at first in the gills and the digestive glands of mussels after exposure to copper (30 mu g l(-1)) or/and thiram (100 mu g l(-1)) for 3 days. The effects of a complex industrial effluent on the antioxidant system were investigated afterwards in a held study: encaged mussels were placed in a river upstream and downstream from a pollution source consisting of the effluent of a cokery for 1 week. These studies demonstrated that in both experimental exposures, the most sensitive parameters were selenium-dependent glutathione peroxidase activity (SeGPx), reduced glutathione levels (GSH) and especially glutathione reductase activity (GRd) which significantly decreased. Decreases in SeGPX and GRd activities were more pronounced in the gills under coexposure to copper and thiram, with 74% and 78% of reduced activity, respectively (P < 0.01); reduction of GSH levels was 50% in this case. In the field study, the activities could be reduced by 35% and 72% in the gills for SeGPx and GRd, respectively; reduction of GSH levels could reach 45%. In contrast, superoxide dismutase and catalase activities remained generally constant in all the treatment groups compared with controls. Lipid peroxidation, as expressed by malonaldehyde content measured by HPLC, was slightly enhanced in experiment with copper+thiram and this increase (from 1.8 to 2.8 fold) was concomitant with a depletion of antioxidant defences. The responses of antioxidant parameters were overall greater in the gills than in the digestive glands of exposed mussels. These results suggest that among antioxidant defence systems, SeGPx, GSH levels and particularly GRd, could represent biomarkers of interest for the estimation of the effects of environmental contamination on freshwater invertebrates. (C) 1997 Elsevier Science B.V.