Preparation of non-fouling surface through the coating with core-polymerized block copolymer micelles having aldehyde-ended PEG shell.
Preparation of non-fouling surface through the coating with core-polymerized block copolymer micelles having aldehyde-ended PEG shell.
复制标题
通过具有醛端PEG壳的核聚合嵌段共聚物胶束的涂层制备防污表面。
DOI：
10.1016/s0927-7765(99)00159-9
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Kataoka
中科院分区：
文献类型：
--
作者：
Emoto;Nagasaki1;Iijima;Kato;Kataoka
采用反应性聚合物胶束进行新型表面改性，以形成防污表面。采用阴离子聚合技术定量合成了PEG末端具有缩醛基团、PLA末端具有甲基丙烯酰基的两亲性聚乙二醇-b-聚(d,l丙交酯)(PEG/PLA)共聚物。由嵌段共聚物制备窄分布的胶束。通过酸处理，将胶束表面的缩醛基定量地转化为醛基。位于胶束核心的甲基丙烯酰基通过自由基聚合而聚合，形成表面具有反应性醛基的核心聚合胶束。将核聚合的反应性胶束涂覆到通过等离子体处理制备的含伯氨基的聚丙烯（PP）板上。采用还原胺化反应通过共价键将反应性核聚合胶束缀合在表面上。通过X射线光电子能谱、δ电位测量和牛血清白蛋白吸附量对涂层进行评价，并与相同条件下的PEG涂层进行比较。 C̱O键与CC̱C键的峰比表明胶束涂层表面的PEG密度高于线性PEG涂层。这也通过 ζ 电位测量得到证实。通过用胶束涂覆氨基-PP表面，δ电势显着降低，而相同条件下的PEG涂层仅明显降低，表明胶束涂层有效地掩盖了表面电荷。此外，胶束覆盖的表面表现出蛋白质吸附的减少。蛋白质吸附的减少以及明显掩盖的表面电荷意味着胶束涂层在生物医学和生物分析应用中的高度适用性。
A new type of surface modification with reactive polymeric micelle was carried out for the creation of non-fouling surface. Amphiphilic poly(ethylene glycol)-b-poly(d,l lactide) (PEG/PLA) copolymers possessing acetal group at PEG-end and methacryloyl group at PLA-end were quantitatively synthesized via an anionic polymerization technique. A micelle of narrow distribution was prepared from the block copolymer. Acetal groups on the micelle surface were quantitatively converted into aldehyde group by an acid treatment. The methacryloyl group located in the core of the micelle was polymerized via radical polymerization to form core-polymerized micelle having reactive aldehyde groups on the surface. The core-polymerized reactive micelle was coated to a primary amino-containing polypropylene (PP) plate that was prepared by a plasma treatment. A reductive amination reaction was employed for a conjugation of the reactive core-polymerized micelle on the surface via a covalent linkage. The coating was evaluated by X-ray photoelectron spectroscopy, ζ-potential measurement, and the adsorption of bovine serum albumin, and compared with the PEG-coating under the same condition. The ratio of peak from C̱O bond to CC̱C bond indicated that the density of PEG on the surface was higher for the micelle coating than the linear PEG-coating. This is also confirmed by the ζ-potential measurement. By coating the amino-PP surface with micelle, the ζ-potential was remarkably decreased while the PEG-coating under the same condition decreased only appreciably, indicating that micelle coating efficiently masked the surface charge. Further, micelle-covered surface exhibited reduction of protein adsorption. The reduction of protein adsorption along with remarkably masked surface charge implies the high applicability of the micelle coatings to biomedical and bioanalytical applications.
影响因子：
10.8
作者：
Yokoyama Masayuki;Miyauchi Mizue;Yamada Noriko;O. Teruo;Sakurai Yasuhisa;Kataoka Kazunori;Inoue Shohei-Inoue-Sh
通讯作者：
Yokoyama Masayuki;Miyauchi Mizue;Yamada Noriko;O. Teruo;Sakurai Yasuhisa;Kataoka Kazunori;Inoue Shohei-Inoue-Sh