Studies on the induction of hydro-cephalus and spongy degeneration by cuprizone feeding and attempts to antidote the toxlcity
Studies on the induction of hydro-cephalus and spongy degeneration by cuprizone feeding and attempts to antidote the toxlcity
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DOI：
10.1016/0024-3205(67)90356-6
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发表时间：
1967-01-01
期刊：
影响因子：
6.1
通讯作者：
CARLTON, WILLIAM W.
中科院分区：
文献类型：
--
作者：
CARLTON, WILLIAM W.
对刚断奶的雄性小鼠饲喂环己酮和双酰肼（铜腙的化学前体），无明显毒性作用。在0.5%铜腙日粮中添加1000倍日粮需要量的硫胺素、维生素B12和叶酸以及利尿剂（Diuril），没有明显的解毒作用。多种维生素溶液、核黄素和维生素A在实验期间降低了死亡率，但没有降低脑积水的发生率或防止海绵变性病变。饲料中130 ppm和260 ppm铜水平可有效降低饲喂0.2%铜唑啉的小鼠脑积水的发病率。补铜对水肿、海绵样变性和星形胶质细胞增生无明显影响。随着开始喂食的年龄增加，喂食铜腙导致小鼠不太可能发生脑积水;喂食8周后，使用5周龄小鼠时脑积水的发生率为100%，使用6周龄小鼠时为75%，喂食8周龄小鼠时为37.5%。0.3%铜腙灌胃5周后小鼠出现脑积水，2周后早期出现海绵变性，4周后大部分脑内观察到胶质细胞增生。髓鞘损失发生较晚，仅在喂食9周的小鼠中观察到。|| 摘要编辑：作者
Weanling male mice were fed cyclohexanone and oxaldihydrazide, chemical precursors of cuprizone, without significant toxic effects. Supplementation of 0.5% cuprizone diets with thiamine, vitamin B12, and folic acid at level of 1000X dietary requirement and the diuretic (Diuril) was without significant antidotal effects. A multiple vitamin solution, riboflavin, and vitamin A reduced mortality over the experimental period but did not reduce the incidence of hydrocephalus or prevent the lesions of spongy degeneration. Copper at levels of 130 ppm and 260 ppm in feed was effective in reducing hydrocephalus incidence in mice fed 0.2% cuprizone. The copper supplements were without effect on the lesions of edema, spongy degeneration and astrogliosis. Mice became less likely to develop hydrocephalus as a result of cuprizone feeding as the age at which feeding began is increased; the incidence of hydrocephalus after 8 weeks of feeding was 100% when 5 week old mice were used, 75% when 6 week old mice were used, and 37.5% when 8 week old mice were fed. Hydrocephalus occurred in mice after 5 weeks of feeding 0.3% cuprizone; spongy degeneration occurred early, after 2 weeks, and glial proliferation was observed in most brains after 4 weeks. Myelin loss occurred late and was noted only in mice fed for 9 weeks. || ABSTRACT AUTHORS: Author