Synthetic peptides used to locate the alpha-bungarotoxin binding site and immunogenic regions on alpha subunits of the nicotinic acetylcholine receptor.
Synthetic peptides used to locate the alpha-bungarotoxin binding site and immunogenic regions on alpha subunits of the nicotinic acetylcholine receptor.
复制标题
用于定位烟碱乙酰胆碱受体的α亚基上的α-银环蛇毒素结合位点和免疫原性区域的合成肽。
DOI：
10.1021/bi00386a004
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Lindstrom,J
中科院分区：
文献类型：
--
作者：
Ralston,S;Sarin,V;Thanh,HL;Rivier,J;Fox,JL;Lindstrom,J
Scott Ralston, 1 Virender Sarin, § Hung Lam Thanh, 1, 11 Jean Rivier, 1 J. Lawrence Fox, § 和 Jon Lindstrom**’1 索尔克生物研究所，圣地亚哥，加利福尼亚州 92138，雅培实验室，雅培公园，北芝加哥，伊利诺伊州 60064，以及生物系，CEN Saclay，91191 Gif法国 sur Yvette Cedex 收到 1986 年 12 月 11 日摘要：已经合成了对应于来自加州鱼雷电器官的乙酰胆碱受体亚基序列的 57% 并从 NH2 延伸到 COOCH 末端的合成肽。通过测定125I-α-银环蛇毒素与合成肽的槽印迹的结合，将变性α亚基上的α-银环蛇毒素结合位点定位在序列al85-199内。进一步的研究表明，序列al90-194中的残基，尤其是半胱氨酸-al92、193，对于结合α-银环蛇毒素至关重要。还原和烷基化研究表明这些半胱氨酸必须通过二硫键连接才能与α-金环蛇毒素结合。通过 125I-肽的间接免疫沉淀来绘制血清抗体与天然受体或亚基的结合位点。鉴定出了几种抗原序列，但没有鉴定出与主要免疫原性区域（高度构象依赖性）相对应的合成肽。
Scott Ralston, 1 Virender Sarin, § Hung Lam Thanh, 1, 11 Jean Rivier, 1 J. Lawrence Fox, § and Jon Lindstrom**’1 The Salk Institute for Biological Studies, San Diego, California 92138, Abbott Laboratories, Abbott Park, North Chicago, Illinois 60064, and Departement de Biologie, CEN Saclay, 91191 Gif sur Yvette Cedex, France Received December 11, 1986 abstract: Synthetic peptides corresponding to 57% of the sequence of a subunits of acetylcholine receptors from Torpedo californica electric organand extending from the NH2 to the COOCH terminus have been synthesized. The a-bungarotoxin binding site on denatured a subunits was mapped within the sequence al85-199 by assaying binding of 125I-a-bungarotoxin to slot blots of synthetic peptides. Further studies showed that residues in the sequence al90-194, especially cysteines-al92, 193, were critical for binding a-bungarotoxin. Reduction and alkylation studies suggested that these cysteines must be disulfide linked for a-bungarotoxinto bind. Binding sites for serum antibodies to native receptors or a subunits were mapped by indirect immunoprecipitation of 125I-peptides. Several antigenic sequences were identified, but a synthetic peptide corresponding to the main immunogenic region (which is highly conformation dependent) was not identified.