Detection of intracellular bacteria in the buds of Scotch fine (Pinus sylvestris L.) by in situ hybridization

Detection of intracellular bacteria in the buds of Scotch fine (Pinus sylvestris L.) by in situ hybridization
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DOI:
10.1128/aem.66.7.3073-3077.2000
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发表时间:
2000-07-01
影响因子:
4.4
通讯作者:
Hohtola, A
Hohtola, A
中科院分区:
生物学2区
文献类型:
--
作者:
Pirttilä, AM;Laukkanen, H;Hohtola, A

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从樟子松(Pinus sylvestris L.)经组织培养鉴定为扭脱甲基杆菌和合黄假单胞菌。采用16SrRNA原位杂交技术研究了松芽中细菌的存在情况。细菌居住在每棵树的芽检查,主要是殖民化的规模原基和树脂导管的细胞。
Bacterial isolates were obtained from pine (Pinus sylvestris L.) tissue cultures and identified as Methylobacterium extorquens and Pseudomonas synxantha. The existence of bacteria in pine buds was investigated by 16S rRNA in situ hybridization. Bacteria inhabited the buds of every tree examined, primarily colonizing the cells of scale primordia and resin ducts.