NGF-potentiating vibsane-type diterpenoids from Viburnum sieboldii.
NGF-potentiating vibsane-type diterpenoids from Viburnum sieboldii.
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DOI：
10.1016/j.bmcl.2010.02.085
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发表时间：
2010-04
影响因子：
2.7
通讯作者：
M. Kubo;Yoshiko. Kishimoto;K. Harada;Hideaki Hioki;Y. Fukuyama
中科院分区：
文献类型：
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作者：
M. Kubo;Yoshiko. Kishimoto;K. Harada;Hideaki Hioki;Y. Fukuyama
利用神经生长因子（NGF）诱导的PC 12细胞，通过生物活性筛选，从天女荚（Viburnum sieboldii）叶中分离得到6个新的二萜类化合物，分别命名为neovibsanin O（1），neovibsanin M（2），neovibsanin L（3），（8 Z）-neovibsanin M（4），15-O-methylvibsanin H（5），5-epi-15-O-methylvibsanin H（6）。化合物1-6的结构经波谱分析和与已报道的vibsane型二萜化合物的核磁共振数据比较确定。化合物3和4以及已知的vibsane型二萜类化合物neovibsanins A（7）和B（8）在20至40μM的浓度范围内显著增强NGF介导的PC 12细胞的神经突生长。
Six new vibsane-type diterpenoids, named neovibsanin O (1), neovibsanin M (2), neovibsanin L (3), (8Z)-neovibsanin M (4), 15-O-methylvibsanin H (5), and 5-epi-15-O-methylvibsanin H (6), were isolated from the leaves of Viburnum sieboldii by bioassay-guided fractionation using NGF-differentiated PC12 cells. The structures of 1–6 were established by analyzing their spectroscopic data and comparing their NMR data with those of previously reported vibsane-type diterpenoids. Compounds 3 and 4, and the known vibsane-type diterpenoids neovibsanins A (7) and B (8) significantly enhanced the neurite outgrowth of NGF-mediated PC12 cells at concentrations ranging from 20 to 40μM.