New insights in integrated response mechanism of Lactobacillus plantarum under excessive manganese stress.
New insights in integrated response mechanism of Lactobacillus plantarum under excessive manganese stress.
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DOI：
10.1016/j.foodres.2017.10.014
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发表时间：
2017-12
影响因子：
8.1
通讯作者：
Y. Tong;Q. Zhai;Wenwei Lu;F. Tian;Jianxin Zhao;Hao Zhang;Wei Chen
中科院分区：
文献类型：
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作者：
Y. Tong;Q. Zhai;Wenwei Lu;F. Tian;Jianxin Zhao;Hao Zhang;Wei Chen
植物乳杆菌作为一种天然的胃肠道寄主，具有保健作用，尤其是去除重金属的作用。系统地研究了植物乳杆菌对过量锰胁迫的生理和蛋白质组学响应机制。分泌更多的胞外聚合物，而细胞更倾向于聚集以抵抗锰胁迫。膜的稳定性是通过上调不饱和脂肪酸的比例来维持的，尤其是环丙烷脂肪酸。细胞内与能量代谢密切相关的天冬氨酸和精氨酸含量在过量锰胁迫下下降。比较2-DE蛋白质组学分析确定了52个在不同水平的锰胁迫下存在显著差异的蛋白质。差异表达的蛋白质涉及碳水化合物、氨基酸、胁迫反应和核苷酸代谢，被归类为抵抗锰胁迫的关键成分。此外，mnth4和mnth5被确定为调节锰运输模型的功能元件。6种关键转运蛋白在不同锰胁迫下的差异表达表明，mnth 1、2和3受MntR负调控，而mnth 4和mnth 5则呈相反的调控模式。综上所述，这项研究为益生菌对类似环境胁迫的反应机制提供了新的见解。
As a natural inhabitant of gastrointestinal tract,Lactobacillus plantarumis well known for its health-promoting effects, especially in heavy metal-removal function. The response mechanism integrated physiology and proteomics ofLactobacillus plantarumunder excessive manganese stress was systematically performed. More extracellular polymeric substance was secreted, whereas cells preferred to aggregate for resisting manganese stress. The stability of the membrane was maintained by up-regulating the proportion of unsaturated fatty acids, especially cyclopropane fatty acids. The contents of intracellular Asp and Arg, closely related with energy metabolism, decreased under excessive manganese stress. Comparative 2-DE proteomic analysis identified 52 proteins that significantly differed under different levels of manganese stress. The differentially expressed proteins, involved in the carbohydrate, amino acid, stress response and nucleotide metabolisms, were categorized as the crucial components during resisting manganese stress. Moreover, MntH 4 and MntH 5 were identified as the functional elements in regulation of manganese transportation model. The differential expression of six key transporter proteins under different manganese stresses indicated that the MntH 1, 2 and 3 was negatively regulated by MntR, while the regulation of MntH 4 and 5 presented the contrary model. Taken altogether, the study provides new insights into the response mechanism of probiotics to similar environmental stress.