High-resolution lipidomics reveals dysregulation of lipid metabolism in respiratory syncytial virus pneumonia mice
High-resolution lipidomics reveals dysregulation of lipid metabolism in respiratory syncytial virus pneumonia mice
复制标题
高分辨率脂质组学揭示呼吸道合胞病毒肺炎小鼠脂质代谢失调
DOI：
10.1039/c8ra05640d
复制
发表时间：
2018-01-01
期刊：
影响因子：
3.9
通讯作者：
Zhao, Xia
中科院分区：
文献类型：
--
作者：
Shan, Jinjun;Qian, Wenjuan;Zhao, Xia
Respiratory syncytial virus (RSV) is a leading viral pathogen responsible for lower respiratory tract infections, particularly in children under five years worldwide, often resulting in hospitalization. At present, the molecular-level interactions between RSV and its host and the underlying mechanisms of RSV-induced inflammation are poorly understood. Herein, we describe an untargeted high-resolution lipidomics platform based on UHPLC-Q-Exactive-MS to assess the lipid alterations of lung tissues and plasma from a mouse model of RSV pneumonia. Untargeted lipidomics using LC-MS with multivariate analysis was applied to describe the lipidomic profiling of the lung tissues and plasma in RSV pneumonia mice. Lipid identification was conducted via an in silico MS/MS LipidBlast library using the MS-DIAL software. We observed distinct compartmental lipid signatures in the mice lung tissues and plasma and significant lipid profile changes between the systematic and localized host responses to RSV. A total of 87 and 68 differential lipids were captured in the mice lung tissue and plasma, respectively, including phospholipids, sphingolipids, acylcarnitine, and fatty acids. Some of these lipids belong to pulmonary surfactants, illustrating that RSV pneumonia-induced aberrations of the pulmonary surfactant system may play a vital role in the etiology of respiratory inflammation. Our findings reveal that the host responses to RSV and various lipid metabolic pathways were linked to disease pathology. Furthermore, our findings could provide mechanistic insights into RSV pneumonia.
