Molecular epidemiology of Impatiens necrotic spot virus on greenhouse ornamental plants in a local area of Japan.
Molecular epidemiology of Impatiens necrotic spot virus on greenhouse ornamental plants in a local area of Japan.
复制标题
日本当地温室观赏植物凤仙花坏死斑病毒的分子流行病学。
DOI：
10.1007/s10327-015-0615-x
复制
发表时间：
2015
影响因子：
1.2
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Nekoduka;S.;Kobayashi;K.;Fuji;S.-i. and Sano;T.
凤仙花坏死斑病毒(Inpatiens Necrotic Spot Virus，INSV)属于冠状病毒属，严重危害温室观赏植物。我们对从岩手县不同温室花卉中分离的INSV进行了群体遗传学分析，以此作为模型来阐明病毒是如何在日本当地入侵和传播的。对采自3个地区7个温室的42个分离物的核衣壳蛋白基因(NG)和基因间隔区(IGR)分别进行了42 30个序列的分析，将其分为10个NG单倍型和13个IGR单倍型。对这两个基因座的联合单倍型分析显示，该地区存在14个NG/IGR单倍型。基于NG序列的INSV群体的遗传结构按地区和温室不同而不同，每个地区主要有3种NG单倍型。基于IGR序列的遗传分析表明，一些温室的群体结构由1个主要的IGR单倍型和一些具有相同NG单倍型的次要单倍型组成。基于NG和IGR序列的系统发育分析表明，INSV单倍型根据地理来源而聚类，包括以前在日本和其他国家报道的分离株。寄主种类似乎不影响INSV种群的遗传结构。这些结果表明，一些方正菌株是从其他疫区通过不同的途径单独传入该地区的各个地区，然后在当地和温室内传播。
Impatiens necrotic spot virus(INSV), belonging to the genus Tospovirus, causes severe damage to greenhouse ornamental plants. We conducted population genetic analyses of INSV isolated from various greenhouse flowers in Iwate Prefecture as a model to elucidate how the virus invaded and spread within a local area of Japan. Forty-two and 30 sequences of the nucleocapsid protein gene (NG) and intergenic regions (IGR), respectively, of the small RNA of the virus were generated from 42 isolates collected from seven greenhouses in three districts, and then they were divided into 10 NG haplotypes and 13 IGR haplotypes. A combined haplotype analysis for the two loci revealed the presence of 14 NG/IGR haplotypes in the region. Genetic structure of the INSV population based on the NG sequences was differentiated according to districts and greenhouses, and 3 major NG haplotypes were predominant in each district. Genetic analysis based on IGR sequences showed that the population structure in some greenhouses consisted of 1 major IGR haplotype and some minor haplotypes with the same NG haplotype. Phylogenetic analysis based on NG and IGR sequences illustrated that INSV haplotypes were clustered according to geographic origin, including the isolates previously reported in Japan and other countries. Host species did not seem to influence the genetic structure of the INSV population. These results suggest that some founder isolates were introduced individually from other epidemic regions to each district in the region through different routes and then spread within the local areas and greenhouses.