In vivo imaging and monitoring of transplanted stem cells: clinical applications.
In vivo imaging and monitoring of transplanted stem cells: clinical applications.
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DOI：
10.1007/s11886-009-0073-1
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发表时间：
2010-01
影响因子：
3.7
通讯作者：
Rodriguez-Porcel, Martin
中科院分区：
文献类型：
--
作者：
Rodriguez-Porcel, Martin
使用干细胞的再生医学已成为冠状动脉疾病的潜在治疗选择，干细胞临床研究目前正在进行中。然而，这些研究的初步结果提供了混合的信息，部分原因是无法将器官功能信息与移植干细胞的存在/不存在相关联。分子生物学和成像技术的最新进展已经允许在活体中成功地非侵入性监测移植的干细胞。在这篇文章中，不同的成像策略（直接标记，间接标记报告基因）研究干细胞的活力和生物学进行了讨论。此外，还讨论了每种方法和成像方式（例如，单光子发射计算机断层扫描、正电子发射断层扫描和MRI）的局限性及其临床使用要求。这些策略的使用将是至关重要的，因为不同的再生疗法正在进行临床使用测试。
Regenerative medicine using stem cells has appeared as a potential therapeutic alternative for coronary artery disease, and stem cell clinical studies are currently on their way. However, initial results of these studies have provided mixed information, in part because of the inability to correlate organ functional information with the presence/absence of transplanted stem cells. Recent advances in molecular biology and imaging have allowed the successful noninvasive monitoring of transplanted stem cells in the living subject. In this article, different imaging strategies (direct labeling, indirect labeling with reporter genes) to study the viability and biology of stem cells are discussed. In addition, the limitations of each approach and imaging modality (eg, single photon emission computed tomography, positron emission tomography, and MRI) and their requirements for clinical use are addressed. Use of these strategies will be critical as the different regenerative therapies are being tested for clinical use.