Localization and inhibition of calcium-activated neutral protease (CANP) in primate skeletal muscle and peripheral nerve.
Localization and inhibition of calcium-activated neutral protease (CANP) in primate skeletal muscle and peripheral nerve.
复制标题
灵长类骨骼肌和周围神经中钙激活中性蛋白酶（CANP）的定位和抑制。
DOI：
10.1016/0014-4886(87)90248-2
复制
发表时间：
1987
影响因子：
5.3
通讯作者：
Stracher,A
中科院分区：
文献类型：
--
作者：
Badalamente,MA;Hurst,LC;Stracher,A
应用间接免疫荧光和过氧化物酶-抗过氧化物酶技术，在光镜和电镜下研究了正常猴（Cebusapella）骨骼肌和周围神经中钙激活中性蛋白酶的定位。在肌肉中，蛋白酶被证明与肌内膜胶原纤维，基膜，肌膜，Z带和神经肌肉接头。在神经中，该酶被证明与神经内膜胶原纤维、基底膜、轴膜以及有髓和无髓轴突的神经丝相关。肌内注射的巯基蛋白酶抑制剂，亮抑酶肽，废除钙激活的中性蛋白酶免疫反应在肌肉和周围神经。这些数据表明，蛋白酶是本地化的细胞内和细胞外的正常灵长类动物的肌肉和周围神经。去神经支配后亮抑酶肽抑制基底膜相关中性蛋白酶可能对保护该结构免受降解并将其作为轴突再生的神经营养晶格而保存具有重要意义。
Localization of calcium-activated neutral protease in normal monkey (Cebus apella) skeletal muscle and peripheral nerve was studied by application of the indirect immunofluorescent and peroxidase-antiperoxidase techniques for light and electron microscopy. In muscle, the protease was demonstrated in association with endomysial collagen fibrils, basal lamina, sarcolemma, and Z-bands and also at neuromuscular junctions. In nerve, the enzyme was demonstrated in association with endoneurial collagen fibrils, basal lamina, axolemma, and neurofilaments of both myelinated and unmyelinated axons. Intramuscular injections of the thiol protease inhibitor, leupeptin, abolished Ca-activated neutral protease immunoreactivity in both muscle and peripheral nerve. These data suggest that the protease is localized both intracellularly and extracellularly in normal primate muscle and peripheral nerve. Inhibition of basal lamina associated neutral protease by leupeptin after denervation may hold significance for protecting this structure against degradation and preserving it as a neurotrophic lattice for axon regeneration.