Occurrence of aflatoxin M(1) in some samples of UHT, raw & pasteurized milk from Indian states of Karnataka and Tamilnadu.
Occurrence of aflatoxin M(1) in some samples of UHT, raw & pasteurized milk from Indian states of Karnataka and Tamilnadu.
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DOI：
10.1016/j.fct.2012.08.034
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
V. Siddappa;D. Nanjegowda;P. Viswanath
中科院分区：
文献类型：
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作者：
V. Siddappa;D. Nanjegowda;P. Viswanath
黄曲霉毒素 M1 (AFM1) 是一种有毒代谢物，存在于食用了受黄曲霉毒素 B1 污染的饲料的哺乳动物的乳汁中。超高温处理 (UHT) 牛奶是一种在印度等发展中国家日益流行的产品，因为这些国家缺乏适当的冷藏或冷藏设施。在这项研究中，使用免疫亲和柱净化样品后，使用荧光检测器，通过反相 HPLC 分析了市场上流行品牌的 45 个 UHT 牛奶样品中是否存在 AFM1。所有原味 UHT 牛奶样本均呈 AFM1 阳性，其中 38% 的 AFM1 含量超过食品法典委员会和该国强制性法规（2011 年 FSSAI 法规）规定的最大允许限值。在 62.5% 的调味 UHT 牛奶中，AFM1 低于可检测水平 (0.02μgL−1)。然而，这些样品中有 12.5% 的含量也超过了最大允许限值。在卡纳塔克邦和泰米尔纳德邦两个州分析的 52 个原奶样品中，AFM1 存在于 61.6%，范围为 0.1–3.8μgL−1。其中17.3%的样品也超出国家监管限量。
Aflatoxin M1(AFM1) is a toxic metabolite found in the milk of lactating animals which have consumed feedstuffs contaminated with aflatoxin B1. Ultra high temperature treated (UHT) milk is a product which is becoming popular in developing countries like India as there is a lack of proper cold storage or refrigeration facilities. In this study, 45 samples of UHT milk of popular brands prevalent in the market were analyzed for the presence of AFM1by reversed phase HPLC using fluorescent detector after cleanup of sample with immunoaffinity columns. All samples of plain UHT milk were positive for AFM1and 38% of these contained levels more than 0.5μg/kg, the maximum permitted limit prescribed by the Codex Alimentarius Commission and by the mandatory regulations of the country, the FSSAI Regulations, 2011. In 62.5% of flavored UHT milk, AFM1was below detectable levels (0.02μgL−1). However, 12.5% of these samples also contained levels exceeding the maximum permitted limits. AFM1was present in 61.6% of the 52 raw milk samples analyzed from the two states of Karnataka and Tamilnadu with a range of 0.1–3.8μgL−1. 17.3% of these samples also exceeded the regulatory limits of the country.