A quantitative morphospace of multicellular organ design in the plant Arabidopsis
A quantitative morphospace of multicellular organ design in the plant Arabidopsis
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DOI：
10.1016/j.cub.2023.09.048
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发表时间：
2023-11-20
期刊：
影响因子：
9.2
通讯作者：
Bassel，George W.
中科院分区：
文献类型：
--
作者：
Duran-Nebreda，Salva;Jackson，Matthew D. B.;Bassel，George W.
器官的功能来自其组成细胞之间的相互作用。细胞组织的研究可以提供深入了解器官功能的结构-功能关系。在这里，我们调查的程度，在细胞组织中的属性可以出现“免费”作为一个新兴的属性嵌入空间中的细胞与那些积极产生的图案化过程。使用三维（3D）数字组织模型建立默认细胞配置。基于网络的分析，这些合成细胞组件建立了一个定量的拓扑基线的细胞组织，授予凭借被动空间包装和最小数量的秩序，出现免费的镶嵌组织。一个3D细胞分辨率的数字组织图谱的模式植物speciesopsis的产生，并在何种程度上在这种生物体中的器官符合默认配置建立通过统计比较与数字组织模型。不同组织中的细胞在不同程度上不符合随机堆积排列。最密切匹配的随机模型是未分化的茎顶端分生组织（SAM），气生器官发出。相比之下，叶和萼片组织表现出最大的偏离这一基线，这表明这些是最“复杂”的组织在拟南芥。对造成这些组织和默认模式之间的差距的模式化原理的研究揭示了细胞伸长和空气空间的引入有助于额外的器官模式化复杂性。这项工作建立了一个定量形态空间，以了解器官构造的原理及其在单个生物体中的多样性。
Organ function emerges from the interactions between their constituent cells. The investigation of cellular organization can provide insight into organ function following structure-function relationships. Here, we investigate the extent to which properties in cellular organization can arise "for free" as an emergent property of embedding cells in space versus those that are actively generated by patterning processes. Default cellular configurations were established using three-dimensional (3D) digital tissue models. Network-based analysis of these synthetic cellular assemblies established a quantitative topological baseline of cellular organization, granted by virtue of passive spatial packing and the minimal amount of order that emerges for free in tessellated tissues. A 3D cellular-resolution digital tissue atlas for the model plant speciesArabidopsiswas generated, and the extent to which the organs in this organism conform to the default configurations was established through statistical comparisons with digital tissue models. Cells in different tissues ofArabidopsisdo not conform to random packing arrangements to varying degrees. Most closely matching the random models was the undifferentiated shoot apical meristem (SAM) from which aerial organs emanate. By contrast, leaf and sepal tissue showed the greatest deviation from this baseline, suggesting these to be the most "complex" tissues inArabidopsis. Investigation of the patterning principles responsible for the gap between these tissues and default patterns revealed cell elongation and the introduction of air spaces to contribute toward additional organ patterning complexity. This work establishes a quantitative morphospace to understand the principles of organ construction and its diversity within a single organism.