Silicon-based microfilters for whole blood cell separation
Silicon-based microfilters for whole blood cell separation
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DOI：
10.1007/s10544-007-9131-x
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发表时间：
2008-04-01
影响因子：
2.8
通讯作者：
Yobas, Levent
中科院分区：
文献类型：
--
作者：
Ji, Hong Miao;Samper, Victor;Yobas, Levent
本文报道了四种主要类型的硅基微滤器用于分离全血中的白色血细胞（WBC）和红细胞（RBC）的比较分析。微过滤器的设计，即堰，柱，错流，和膜，都施加了相同的截止尺寸为3.5 μ m，选择性地捕获白细胞。使用人全血，对微滤器的血液处理能力、WBC捕获效率和RBC通过效率进行了表征和比较。基于实验结果，错流微过滤器是上级的，并且可以与下游组件集成用于芯片上基因组分析。
This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 mu m to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.