Detection of induced DNA strand breaks with improved sensitivity in human cells.
Detection of induced DNA strand breaks with improved sensitivity in human cells.
复制标题
检测人类细胞中诱导的 DNA 链断裂，灵敏度更高。
作者：
B. Rydberg
当用不同放射性核素标记的两个细胞群的混合物用DNA解绕技术进行测试时，可以对DNA链断裂进行准确的比较。在辐照细胞和对照细胞的混合物中，通过该技术可以检测到低至1rad剂量的影响。这相当于每个细胞10至20个DNA单链断裂，或每个染色体少于一个断裂。
When mixtures of two cell populations labeled with different radionuclides are tested with DNA unwinding technique, an accurate comparison with regard to DNA strand breaks can be made. In mixtures of irradiated and control cells, effects were detected by this technique down to a dose of 1 rad. This corresponds to 10 to 20 DNA single-strand breaks per cell, or less than one break per chromosome.