Metal ion dependence, thermodynamics, and kinetics for intramolecular docking of a GAAA tetraloop and receptor connected by a flexible linker
Metal ion dependence, thermodynamics, and kinetics for intramolecular docking of a GAAA tetraloop and receptor connected by a flexible linker
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DOI：
10.1021/bi0520941
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发表时间：
2006-03-21
期刊：
影响因子：
2.9
通讯作者：
Pardi, A
中科院分区：
文献类型：
--
作者：
Downey, CD;Fiore, JL;Pardi, A
GAAA四环受体基序是RNA中常见的三级相互作用。该基序通常与复杂RNA结构中的其他三级相互作用组合出现。因此，很难直接测量单个GAAA四环-受体相互作用对RNA折叠特性的贡献。为了研究分离的相互作用的动力学和热力学，GAAA四环结构域和受体结构域通过单链A（7）接头连接。荧光共振能量转移（FRET）实验被用来探测GAAA四环和受体的分子内对接。使用多种金属离子诱导对接，其中离子的电荷是确定促进对接所需的离子浓度的最重要因素{[Co（NH3）（6）（3+）（]）
The GAAA tetraloop-receptor motif is a commonly occurring tertiary interaction in RNA. This motif usually occurs in combination with other tertiary interactions in complex RNA structures. Thus, it is difficult to measure directly the contribution that a single GAAA tetraloop-receptor interaction makes to the folding properties of a RNA. To investigate the kinetics and thermodynamics for the isolated interaction, a GAAA tetraloop domain and receptor domain were connected by a single-stranded A(7) linker. Fluorescence resonance energy transfer (FRET) experiments were used to probe intramolecular docking of the GAAA tetraloop and receptor. Docking was induced using a variety of metal ions, where the charge of the ion was the most important factor in determining the concentration of the ion required to promote docking {[Co(NH3)(6)(3+)(])