Live transportation of Macrobrachium rosenbergii (De Man) in chilled sawdust
Live transportation of Macrobrachium rosenbergii (De Man) in chilled sawdust
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DOI：
10.1111/j.1365-2109.2005.01245.x
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发表时间：
2005-02-15
影响因子：
2
通讯作者：
Salin, KR
中科院分区：
文献类型：
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作者：
Salin, KR
在有通风的冷藏槽中，对养殖罗氏沼虾（45-52 g）从25℃低温麻醉至15℃低温麻醉，分别在8 h内（慢，T(1)）、4 h内（中，T(2)）、2 h内（快，T(3)）分别以1.26+/-0.09℃h（-1）、1.52 +/-0.36℃h（-1）三种冷却速率进行了试验。经过不同冷却速率冷麻醉的对虾被包装在两层潮湿和冷冻（2-3度）木屑之间的绝缘纸板箱中（3个），并在15+/-1度的冷藏柜中保存，设定持续时间为6、9、12、15和18小时。通过在初始温度为20度的充气水中恢复对虾的活力来确定其存活率。在3小时内升高到29+/-1度。实验用适应盐度为12 g L（-1）的微咸水的浆果雌性重复进行，并以3小时的间隔记录活存6至24小时后的存活率。在三种测试的冷却速率下，使用概率分析确定冷麻醉和活存对虾获得100%存活率的统计有效安全持续时间为7.39。在T(1)、T(2)和T(3)中，成对虾为6.98和4.54 h，雌对虾为7.87、8.17和6.43 h。在T(1)、T(2)和T(3)中，成虾的存活率分别为16.47、12.14和8.35 h，雌虾的存活率分别为18.49、19.02和11.11 h。将活存后恢复活力的浆果对虾在水箱中孵育，并饲养至幼虫后（PL-5），并与对照进行比较。处理与对照的幼虫孵化繁殖力、成活率和PL L（-1）产量均无显著差异，说明冷麻醉和活存法可用于成功的运虾。
Three cooling rates of 1.26+/-0.09degreesC h(-1) within 8 h (slow, T(1)), 2.52+/-0.18degreesC h(-1) within 4 h (moderate, T(2)) and 5.04+/-0.36degreesC h(-1) within 2 h (fast, T(3)) were tested to cold-anaesthetize farm raised Macrobrachium rosenbergii (De Man) (45-52 g) in each case from 25degreesC down to 15+/-1degreesC in a refrigerated chilling tank, provided with aeration. The cold-anaesthetized prawns subjected to each chilling rate were packed in an insulated cardboard box (triplicate) between two layers of moist and chilled (2-3degreesC) sawdust, and kept inside a chilled storage cabinet at 15+/-1degreesC, for set durations of 6, 9, 12, 15 and 18 h. Survival was determined by revitalizing the prawns in aerated water with an initial temperature of 20degreesC, which was raised to 29+/-1degreesC within 3 h. The experiment was repeated using berried females acclimated to brackishwater of 12 g L(-1) salinity and the percentage survival recorded after live storage for durations ranging from 6 to 24 h at intervals of 3 h. Statistically valid safe durations for obtaining 100% survival of the cold anaesthetized and live stored prawns were determined using probit analysis at the three chilling rates tested, and were found to be 7.39, 6.98 and 4.54 h in the case of adult prawns, and 7.87, 8.17 and 6.43 h for berried females for T(1), T(2) and T(3) respectively. For practical purposes, the durations that yielded 95% survival rates were computed to be 16.47, 12.14 and 8.35 h in the case of adult prawns and 18.49, 19.02 and 11.11 h for berried females for T(1), T(2), and T(3) respectively. The berried prawns revitalized after live storage were incubated in tanks and the zoea larvae reared up to postlarvae (PL-5), and compared against a control. No significant difference was found in larval hatch fecundity, survival rate and the production of PL L(-1) between the treatment and control, indicating that the method of cold anaesthetization and live storage of berried prawns could be used for successful transportation of broodstock.