B. subtilis CNBG-PGPR-1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato.
B. subtilis CNBG-PGPR-1 induces methionine to regulate ethylene pathway and ROS scavenging for improving salt tolerance of tomato.
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枯草杆菌CNBG-PGPR-1通过诱导蛋氨酸调节乙烯途径和清除ROS来提高番茄的耐盐性。
DOI：
10.1111/tpj.16489
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发表时间：
2023-10
期刊：
影响因子：
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通讯作者：
Liuchun Feng;Qi Li;Dongqin Zhou;Mingyun Jia;Zhuangzhuang Liu;Zhaoqi Hou;Quanjin Ren;Shengdong Ji
中科院分区：
文献类型：
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作者：
Liuchun Feng;Qi Li;Dongqin Zhou;Mingyun Jia;Zhuangzhuang Liu;Zhaoqi Hou;Quanjin Ren;Shengdong Ji
土壤盐分严重威胁植物生长和作物产量。PGPR的利用是提高植物耐盐性的有效策略，但其作用机制尚未见报道。在本研究中，我们研究了枯草芽孢杆菌CNBG-PGPR-1在提高植物耐盐性方面的作用，并阐明了相关的分子途径。结果表明，CNBG-PGPR-1显著改善了番茄叶片的细胞动态平衡和光合作用效率，减轻了盐胁迫引起的离子毒害和渗透胁迫。转录组分析发现，CNBG-PGPR-1通过激活复杂的分子途径提高植物的耐盐性，其中植物激素信号转导起重要作用。比较分析和药理实验证实，乙烯途径与CNBG-PGPR-1提高植物耐盐性的有益作用密切相关。此外，我们还发现作为乙烯合成前体的蛋氨酸在番茄对CNBG-PGPR-1的响应中显著积累。外源L蛋氨酸在很大程度上模拟了CNBG-PGPR-1的有益效应，并激活了乙烯途径相关基因的表达，表明CNBG-PGPR-1诱导蛋氨酸积累调节番茄的乙烯途径。最后，CNBG-PGPR-1通过激活ROS清道夫编码基因来降低盐诱导的ROS，这些基因主要涉及GSH代谢和POD相关基因，它们也与蛋氨酸代谢密切相关。总之，我们的研究表明，CNBG-PGPR-1诱导的蛋氨酸是通过乙烯途径和清除ROS来提高植物耐盐性的关键调节因子，这为有益微生物提高植物耐盐性的机制提供了新的理解。
Soil salinity severely threatens plant growth and crop yields. The utilization of PGPR is an effective strategy for enhancing plant salt tolerance, but the mechanisms involved in this process have rarely been reported. In this study, we investigated the effects of Bacillus subtilis CNBG-PGPR-1 on improving plant salt tolerance and elucidated the molecular pathways involved. The results showed that CNBG-PGPR-1 significantly improved the cellular homeostasis and photosynthetic efficiency of leaves and reduced ion toxicity and osmotic stress caused by salt in tomato. Transcriptome analysis uncovered that CNBG-PGPR-1 enhanced plant salt tolerance through the activation of complex molecular pathways, with plant hormone signal transduction playing an important role. Comparative analysis and pharmacological experiments confirmed that the ethylene pathway was closely related to the beneficial effect of CNBG-PGPR-1 on improving plant salt tolerance. Furthermore, we found that methionine, a precursor of ethylene synthesis, significantly accumulated in response to CNBG-PGPR-1 in tomato. Exogenous L-methionine largely mimicked the beneficial effects of CNBG-PGPR-1 and activated the expression of ethylene pathway-related genes, indicating CNBG-PGPR-1 induces methionine accumulation to regulate the ethylene pathway in tomato. Finally, CNBG-PGPR-1 reduced salt-induced ROS by activating ROS scavenger-encoding genes, mainly involved in GSH metabolism and POD-related genes, which were also closely linked to methionine metabolism. Overall, our studies demonstrate that CNBG-PGPR-1-induced methionine is a key regulator in enhancing plant salt tolerance through the ethylene pathway and ROS scavenging, providing a novel understanding of the mechanism by which beneficial microbes improve plant salt tolerance.