Knockout of NCOA5 impairs proliferation and migration of hepatocellular carcinoma cells by suppressing epithelial-tomesenchymal transition
Knockout of NCOA5 impairs proliferation and migration of hepatocellular carcinoma cells by suppressing epithelial-tomesenchymal transition
复制标题
NCOA5 的敲除通过抑制上皮-间质转化来损害肝细胞癌细胞的增殖和迁移
DOI：
10.1016/j.bbrc.2018.04.017
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发表时间：
2018-06-02
影响因子：
3.1
通讯作者：
Luo, Rongcheng
中科院分区：
文献类型：
--
作者：
He, Jingcai;Zhang, Wan;Luo, Rongcheng
核受体辅激活因子5（NCOA 5）在多种恶性肿瘤的发生发展中起重要作用。然而，潜在的机制仍然不清楚。在本研究中，我们通过CRISPR/Cas9介导的基因组编辑成功地产生了NCOA 5敲除的肝细胞癌（HCC）细胞，发现NCOA 5敲除显著抑制HCC细胞的增殖和肿瘤微球形成。此外，NCOA 5敲除的HCC细胞的迁移能力下降。机制分析表明，敲除NCOA 5可以抑制肝癌细胞的上皮间质转化（EMT）。总之，我们的研究结果为NCOA 5在HCC进展中的作用提供了一个机制性的见解。(C)2018爱思唯尔公司All rights reserved.
Nuclear receptor coactivator 5 (NCOA5) plays important roles in the development of a variety of malignancies. However, the underlying mechanisms remain obscure. In this study, we successfully generated the NCOA5 knockout hepatocellular carcinoma (HCC) cells by CRISPR/Cas9 - mediated genome editing and found that knockout of NCOA5 inhibited the proliferation and tumor microsphere formation of HCC cells significantly. Moreover, the migration ability of NCOA5 knockout HCC cells declined. Mechanistic analyses indicated that knockout of NCOA5 can suppress the epithelial - mesenchymal transition (EMT) in HCC cells. In conclusion, our findings provide a mechanistic insight into the role of NCOA5 in HCC progression. (C) 2018 Elsevier Inc. All rights reserved.