A pilot scale two-stage anaerobic digester treating food waste leachate (FWL): Performance and microbial structure analysis using pyrosequencing
A pilot scale two-stage anaerobic digester treating food waste leachate (FWL): Performance and microbial structure analysis using pyrosequencing
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DOI：
10.1016/j.procbio.2013.10.022
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发表时间：
2014-02-01
影响因子：
4.4
通讯作者：
Um, Youngsoon
中科院分区：
文献类型：
--
作者：
Kim, Seil;Bae, Jaesang;Um, Youngsoon
韩国食品垃圾回收设施产生的食品垃圾渗滤液（FWL）是一个严重的环境问题。在实验室规模内对 FWL 的厌氧消化进行了大量研究；然而，关于大规模厌氧消化系统（ADS）的信息很少。在本研究中，使用 FWL 操作中试规模的两相 ADS，并使用焦磷酸测序研究了 ADS 性能和微生物结构动力学。使用含有高浓度挥发性脂肪酸 (12,435 +/- 2203 mg/L) 的 FWL，ADS 运行了 136 天，挥发性酸 (VS) 去除效率为 74-89%，CH4 产量为 0.39-0.85 Nm(3)/kg 还原 VS。 76、101 和 132 天的微生物结构表明产甲烷菌种群从乙酰分解产甲烷菌（Methanosarcina 和 Methanosaeta）转变为氢营养产甲烷菌（Methanobacter 和 Methanoculleus）。细菌群落也转移到与氢营养产甲烷菌（梭菌）互养相关的分类单元。统计分析发现VS去除率与甲烷八叠球菌呈正相关，与甲烷杆菌呈负相关。这里提出的结果表明，在中试规模的 ADS 系统中，乙酰分解产甲烷菌及其相关细菌对于去除 VS 更有效，为大规模 ADS 中的 FWL 处理提供了有用的信息。 (C) 2013 Elsevier Ltd. 保留所有权利。
Food waste leachate (FWL) from the food waste recycling facilities in Korea is a serious environmental problem. Much research was done on anaerobic digestion of FWL in a lab-scale; however, there is little information on a large scale anaerobic digestion system (ADS). In this study, a two-phase ADS in a pilot scale was operated using FWL and the ADS performance and microbial structure dynamics using pyrosequencing were investigated. The ADS was operated for 136 days using FWL containing a high concentration of volatile fatty acid (12,435 +/- 2203 mg/L), exhibiting volatile acid (VS) removal efficiency of 74-89% and CH4 yield of 0.39-0.85 Nm(3)/kg of reduced VS. The microbial structure at 76, 101, and 132 days indicated the methanogen population shift from acetoclastic methanogens (Methanosarcina and Methanosaeta) to hydrogenotrophic methanogens (Methanobacterium and Methanoculleus). The bacterial community also shifted to the taxa syntrophically related with hydrogenotrophic methanogens (Clostridia). The statistical analysis revealed the positive correlation of VS removal efficiency with Methanosarcina, but the negative correlation with Methanobacterium. The results presented here suggest that acetoclastic methanogens and their associated bacteria were more efficient for VS removal in the pilot scale ADS system, providing useful information for FWL treatment in a large scale ADS. (C) 2013 Elsevier Ltd. All rights reserved.