H2Bub1 Regulates RbohD-Dependent Hydrogen Peroxide Signal Pathway in the Defense Responses to Verticillium dahliae Toxins
H2Bub1 Regulates RbohD-Dependent Hydrogen Peroxide Signal Pathway in the Defense Responses to Verticillium dahliae Toxins
复制标题
H2Bub1 在对大丽黄萎病毒素的防御反应中调节 RbohD 依赖性过氧化氢信号通路
DOI：
10.1104/pp.19.00913
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Li Yingzhang
中科院分区：
文献类型：
--
作者：
Zhao Jun;Chen Qiuhong;Zhou Sa;Sun Yuhui;Li Xinyue;Li Yingzhang
组蛋白H_2B单素化(H_2Bub1)在拟南芥的生长发育和逆境反应中起着重要的调节作用。在本研究中，我们利用野生型和HUB1和HUB2功能丧失的拟南芥植株来阐明植物对黄萎病毒素(VD-毒素)的防御反应的调节机制。我们证明了HUB介导的H_2Bub1通过促进组蛋白H3三甲基化在Lys-4上的富集组蛋白H3来调节NADPH氧化酶RbohD的表达，以响应VD毒素。RbohD依赖的过氧化氢(H_2O_2)信号是对VD毒素防御反应中的关键调节器。此外，H_2Bub1还影响转录后丝裂原活化蛋白激酶(MPK)信号转导。MPK3和MPK6的激活需要H2Bub1。MPK3和MPK6参与调节RbohD介导的过氧化氢的产生。MPK3和MPK6与蛋白酪氨酸磷酸酶(PTPs)相关，如酪氨酸特异性磷酸酶1和丝裂原活化蛋白激酶磷酸酶1，它们负向调节过氧化氢的产生。此外，H_2Bub1参与了WRKY33的表达调控。WRKY33直接与RbohD启动子结合，作为转录因子调控RbohD的表达。综上所述，我们的结果表明，在拟南芥中，H_2Bub1调节依赖于RbohD的H_2O_2的产生，而PTP-MPK3/6-WRKY通路在调节依赖于RbohD的H_2O_2信号对Vd毒素的防御反应中起重要作用。
Histone H2B monoubiquitination (H2Bub1) plays critical roles in regulating growth and development as well as stress responses in Arabidopsis (Arabidopsis thaliana). In this study, we used wild-type and HUB1 and HUB2 loss-of-function Arabidopsis plants to elucidate the mechanisms involved in the regulation of the plant’s defense responses toVerticillium dahliaetoxins (Vd-toxins). We demonstrated that HUB-mediated H2Bub1 regulates the expression of the NADPH oxidaseRbohDby enhancing the enrichment of histone H3 trimethylated on Lys-4 in response to Vd-toxins.RbohD-dependent hydrogen peroxide (H2O2) signaling is a critical modulator in the defense response against Vd-toxins. Moreover, H2Bub1 also affects posttranscriptional mitogen-activated protein kinase (or MPK) signaling. H2Bub1 was required for the activation of MPK3 and MPK6. MPK3 and MPK6 are involved in regulatingRbohD-mediated H2O2production. MPK3 and MPK6 are associated with protein tyrosine phosphatases (PTPs), such as Tyr-specific phosphatase1 and mitogen-activated protein kinases phosphatase1, which negatively regulated H2O2production. In addition, H2Bub1 is involved in regulating the expression ofWRKY33. WRKY33 directly binds toRbohDpromoter and functions as a transcription factor to regulate the expression ofRbohD. Collectively, our results indicate that H2Bub1 regulates the NADPH oxidaseRbohD-dependent H2O2production and that the PTP-MPK3/6-WRKY pathway plays an important role in the regulation ofRbohD-dependent H2O2signaling in defense responses to Vd-toxins in Arabidopsis.