Selective decrease of bis(monoacylglycero)phosphate content in macrophages by high supplementation with docosahexaenoic acid
Selective decrease of bis(monoacylglycero)phosphate content in macrophages by high supplementation with docosahexaenoic acid
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DOI：
10.1194/jlr.m800300-jlr200
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发表时间：
2009-02-01
影响因子：
6.5
通讯作者：
Delton-Vandenbroucke, Isabelle
中科院分区：
文献类型：
--
作者：
Bouvier, Jerome;Berry, Karin A. Zemski;Delton-Vandenbroucke, Isabelle
双（单酰基甘油）磷酸（BMP）是一种独特的磷脂（PL），优先发现于晚期内体膜，在那里它形成专门的脂质结构域。最近，使用培养的巨噬细胞与抗BMP抗体处理，我们表明，BMP丰富的结构域参与胆固醇稳态。我们以前曾强调过，与其他PUFA相比，BMP更容易积累二十二碳六烯酸（DHA）。因为磷脂酰甘油（PG）被报道为BMP合成的RAW巨噬细胞的前体，我们研究了PG补充对FA组合物和BMP在该细胞系中的量的影响。补充二油酰-PG（18：1/18：1-PG）诱导BMP积累，同时增加油酸比例。补充高浓度的二十二碳六烯酰-PG（22：6/22：6-PG）导致BMP中DHA的显著富集，导致diDHA分子种类的形成。然而，BMP的量被选择性地减少。在补充高浓度的非酯化DHA后观察到类似的效果。维生素E的添加阻止了BMP的下降，并进一步增加了其DHA含量。补充22：6/22：6-PG可促进BMP的积累，并提高22：6/22：6-BMP的比例。富含DHA的BMP细胞暴露于氧化剂条件后显着降解，相反，富含油酸的BMP，这是不受影响。使用无细胞系统，我们表明，22：6/22：6-BMP是高度氧化和部分保护胆固醇氧化，与18：1/18：1-BMP相比。我们的数据表明，BMP中的高DHA含量导致该PL的特定降解，可能是通过diDHA分子种类，其非常容易过氧化，因此，在其附近是潜在的抗氧化剂。- Bouvier，J.，K. A. Zemski Berry，F. Hullin Matsuda，A. Makino，S. Michaud，A.热卢恩河C. Murphy，T.小林，M.拉加德和我戴尔顿-范登布鲁克高剂量补充二十二碳六烯酸可选择性降低巨噬细胞中双（单酰基甘油）磷酸含量。J. Lipid Res. 2009. 50：243-255。
Bis(monoacylglycero) phosphate (BMP) is a unique phospholipid (PL) preferentially found in late endosomal membranes, where it forms specialized lipid domains. Recently, using cultured macrophages treated with anti-BMP antibody, we showed that BMP-rich domains are involved in cholesterol homeostasis. We had previously stressed the high propensity of BMP to accumulate docosahexaenoic acid (DHA), compared with other PUFAs. Because phosphatidylglycerol (PG) was reported as a precursor for BMP synthesis in RAW macrophages, we examined the effects of PG supplementation on both FA composition and amount of BMP in this cell line. Supplementation with dioleoyl-PG (18:1/18:1-PG) induced BMP accumulation, together with an increase of oleate proportion. Supplementation with high concentrations of didocosahexaenoyl-PG (22:6/22:6-PG) led to a marked enrichment of DHA in BMP, resulting in the formation of diDHA molecular species. However, the amount of BMP was selectively decreased. Similar effects were observed after supplementation with high concentrations of nonesterified DHA. Addition of vitamin E prevented the decrease of BMP and further increased its DHA content. Supplementation with 22: 6/22: 6-PG promoted BMP accumulation with an enhanced proportion of 22:6/22:6-BMP. DHA-rich BMP was significantly degraded after cell exposure to oxidant conditions, in contrast to oleic acid-rich BMP, which was not affected. Using a cell-free system, we showed that 22: 6/22: 6-BMP is highly oxidizable and partially protects cholesterol oxidation, compared with 18:1/18:1-BMP. Our data suggest that high DHA content in BMP led to specific degradation of this PL, possibly through the diDHA molecular species, which is very prone to peroxidation and, as such, a potential antioxidant in its immediate vicinity. - Bouvier, J., K. A. Zemski Berry, F. Hullin Matsuda, A. Makino, S. Michaud, A. Geloen, R. C. Murphy, T. Kobayashi, M. Lagarde, and I. Delton-Vandenbroucke. Selective decrease of bis(monoacylglycero)phosphate content in macrophages by high supplementation with docosahexaenoic acid. J. Lipid Res. 2009. 50: 243-255.