The effects of early exercise on brain damage and recovery after focal cerebral infarction in rats
The effects of early exercise on brain damage and recovery after focal cerebral infarction in rats
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DOI：
10.1111/j.1748-1716.2010.02174.x
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发表时间：
2011-02-01
影响因子：
6.3
通讯作者：
Yoshida, Y.
中科院分区：
文献类型：
--
作者：
Matsuda, F.;Sakakima, H.;Yoshida, Y.
目的：运动可增强大鼠脑卒中后的神经可塑性，促进运动恢复。本研究旨在探讨跑台运动是否能减轻脑损伤，增强中期因子（MK）和神经生长因子（NGF）的表达，促进血管新生，降低caspase-3的表达。方法：77只Wistar大鼠随机分为3组（缺血对照组36只，缺血运动组36只，假运动组5只）。中风是由90分钟的左大脑中动脉闭塞使用管腔内细丝。从第二天开始，使大鼠在跑步机上跑步20分钟，每天一次，最多连续28天。缺血后的功能恢复进行了评估，使用梁行走试验和神经系统评价量表在所有大鼠。在诱导缺血后第1、3、5、7、14和28天，对脑体积和MK、NGF、抗血小板内皮细胞粘附分子（PECAM-1）和caspase-3的表达进行评价。运动组运动28天后梗死体积（12.4 ± 0.8%）较对照组（19.8 ± 4.2%）明显减少（P < 0.01）。MK，神经生长因子和PECAM-1的细胞表达水平显着增加，而caspase-3的减少，在周围梗死区的exercisedrats.Conclusions：我们的研究结果表明，跑步机运动改善运动行为，减少神经功能缺损和梗死体积，这表明它可能有助于恢复中枢神经系统损伤。
Aim:Exercise can be used to enhance neuroplasticity and facilitate motor recovery after a stroke in rats. We investigated whether treadmill running could reduce brain damage and enhance the expression of midkine (MK) and nerve growth factor (NGF), increase angiogenesis and decrease the expression of caspase-3.Methods:Seventy-seven Wistar rats were split into three experimental groups (ischaemia-control: 36, ischaemia-exercise: 36, sham-exercise: 5). Stroke was induced by 90-min left middle cerebral artery occlusion using an intraluminal filament. Beginning on the following day, the rats were made to run on a treadmill for 20 min once a day for a maximum of 28 consecutive days. Functional recovery after ischaemia was assessed using the beam-walking test and a neurological evaluation scale in all rats. Infarct volume, and the expression of MK, NGF, anti-platelet-endothelial cell adhesion molecule (PECAM-1), and caspase-3 were evaluated at 1, 3, 5, 7, 14 and 28 days after the induction of ischaemia.Results:Over time motor coordination and neurological deficits improved more in the exercised group than in the non-exercised group. The infarct volume in the exercised group (12.4 +/- 0.8%) subjected to treadmill running for 28 days was significantly decreased compared with that in the control group (19.8 +/- 4.2%, P < 0.01). The cellular expression levels of MK, NGF and PECAM-1 were significantly increased while that of caspase-3 was decreased in the peri-infarct area of the exercised rats.Conclusions:Our findings show that treadmill exercise improves motor behaviour and reduces neurological deficits and infarct volume, suggesting that it may aid recovery from central nervous system injury.