Detection and genome sequencing of porcine circovirus 3 in neonatal pigs with congenital tremors in South China
Detection and genome sequencing of porcine circovirus 3 in neonatal pigs with congenital tremors in South China
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DOI：
10.1111/tbed.12702
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发表时间：
2017-12-01
影响因子：
4.3
通讯作者：
Ma, J. Y.
中科院分区：
文献类型：
--
作者：
Chen, G. H.;Mai, K. J.;Ma, J. Y.
猪圆环病毒3型（Porcine Circovirus 3，PCV 3）是一种新型圆环病毒，在美国首次发现于患有猪皮炎和肾病综合征、繁殖障碍、心脏和多系统炎症的仔猪和母猪中。在此，首次从华南地区具有先天性震颤（CT）临床体征的新生猪中鉴定出7株PCV 3菌株。采用实时荧光定量PCR技术分析了猪圆环病毒3型的组织嗜性，结果表明，猪圆环病毒3型在猪的脑和心脏中检测到高载量的病毒基因组。7株新分离的PCV 3与美国和中国报道的11株PCV 3的核苷酸同源性为96.8%~ 99.6%。基于全基因组序列的系统发育分析表明，所有PCV 3株聚在一起，并与其他圆环病毒种明确分开。本研究报告了首次在CT感染的新生仔猪中鉴定出PCV 3，并提供了中国广东和广西两省CT感染新生仔猪的流行病学信息。
Porcine circovirus 3 (PCV3) is a novel circovirus first discovered in the United States in piglets and sows with porcine dermatitis and nephropathy syndrome, reproductive failure, cardiac and multisystemic inflammation. Here, seven PCV3 strains were identified for the first time from neonatal pigs with clinical signs of congenital tremors (CT) in South China. The tissue tropism of PCV3 in CT-affected piglets was analysed by the real-time quantitative PCR, and the result showed that high loads of viral genomes were detected in the brains and hearts. The complete genomes of seven new PCV3 revealed 96.8%-99.6% nucleotide identities with eleven other PCV3 strains previously reported from the United States and China. Phylogenetic analysis based on the complete genome sequences showed that all PCV3 strains clustered together and were clearly separated from other circovirus species. This study reports on the first identification of PCV3 in CT-affected newborn piglets and provides the epidemiological information of neonatal piglets with CT in Guangdong and Guangxi Provinces of China.