In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis.
In vivo bioluminescence imaging and histopathopathologic analysis reveal distinct roles for resident and recruited immune effector cells in defense against invasive aspergillosis.
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DOI：
10.1186/1471-2180-10-105
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发表时间：
2010-04-08
期刊：
影响因子：
4.2
通讯作者：
Brock M
中科院分区：
文献类型：
--
作者：
Ibrahim-Granet O;Jouvion G;Hohl TM;Droin-Bergère S;Philippart F;Kim OY;Adib-Conquy M;Schwendener R;Cavaillon JM;Brock M
Invasive aspergillosis (IA) is a major cause of infectious morbidity and mortality in immune compromised patients. Studies on the pathogenesis of IA have been limited by the difficulty to monitor disease progression in real-time. For real-time monitoring of the infection, we recently engineered a bioluminescent A. fumigatus strain. In this study, we demonstrate that bioluminescence imaging can track the progression of IA at different anatomic locations in a murine model of disease that recapitulates the natural route of infection. To define the temporal and functional requirements of distinct innate immune cellular subsets in host defense against respiratory A. fumigatus infection, we examined the development and progression of IA using bioluminescence imaging and histopathologic analysis in mice with four different types of pharmacologic or numeric defects in innate immune function that target resident and recruited phagocyte subsets. While bioluminescence imaging can track the progression and location of invasive disease in vivo, signals can be attenuated by severe inflammation and associated tissue hypoxia. However, especially under non-inflammatory conditions, such as cyclophosphamide treatment, an increasing bioluminescence signal reflects the increasing biomass of alive fungal cells. Imaging studies allowed an in vivo correlation between the onset, peak, and kinetics of hyphal tissue invasion from the lung under conditions of functional or numeric inactivation of phagocytes and sheds light on the germination speed of conidia under the different immunosuppression regimens. Conditions of high inflammation -either mediated by neutrophil influx under corticosteroid treatment or by monocytes recruited during antibody-mediated depletion of neutrophils- were associated with rapid conidial germination and caused an early rise in bioluminescence post-infection. In contrast, 80% alveolar macrophage depletion failed to trigger a bioluminescent signal, consistent with the notion that neutrophil recruitment is essential for early host defense, while alveolar macrophage depletion can be functionally compensated.
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DOI：
10.1086/600380
发表时间：
2009-08-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Mircescu MM;Lipuma L;van Rooijen N;Pamer EG;Hohl TM
通讯作者：
Hohl TM
DOI：
10.1086/591969
发表时间：
2008-10-15
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Garcia-Vidal C;Upton A;Kirby KA;Marr KA
通讯作者：
Marr KA
影响因子：
3.1
作者：
Ibrahim-Granet, O;Philippe, B;Latgé, JP
通讯作者：
Latgé, JP
影响因子：
4.1
作者：
Murata, M;Ohnishi, S;Kawanishi, S
通讯作者：
Kawanishi, S
影响因子：
2.8
作者：
HUITINGA, I;DAMOISEAUX, JGMC;DIJKSTRA, CD
通讯作者：
DIJKSTRA, CD