Direct electron transfer and electrocatalysis of hemoglobin adsorbed on mesoporous carbon through layer-by-layer assembly.
Direct electron transfer and electrocatalysis of hemoglobin adsorbed on mesoporous carbon through layer-by-layer assembly.
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DOI：
10.1016/j.bios.2006.07.022
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发表时间：
2007-03
影响因子：
12.6
通讯作者：
Jiu-Ju Feng;Jingjuan Xu;Hongyuan Chen
中科院分区：
文献类型：
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作者：
Jiu-Ju Feng;Jingjuan Xu;Hongyuan Chen
利用壳聚糖作为CMK-3与玻碳电极表面的有效连接剂，通过层层组装的方法构建了多层膜修饰电极。用扫描电子显微镜表征了{Hb/CMK-3}N膜的形貌，并用UV-Vis光谱和电化学方法研究了Hb与CMK-3的相互作用。在最佳条件下，{HB/CMK-3}6薄膜在pH 7.0的缓冲液中，在约−377和−296 mV处有一对稳定且清晰的氧化还原峰。此外，{HB/CMK-3}6薄膜对H_2O_2和O_2的还原均表现出良好的电催化性能。基于所形成的膜及其直接电子转移行为，提出了一种新型的测定H_2O_2的生物传感器，在S/N=3.时，检测范围为1.2~57μM，检出限为0.6μM。CMK-3为蛋白质固定化和生物传感器的制备提供了理想的基质。
Using chitosan as an effective linker between CMK-3 and glassy carbon electrode surface, {Hb/CMK-3}nmultilayer film-modified electrodes were constructed through layer-by-layer assembly. The morphology of thus-formed {Hb/CMK-3}nfilm was characterized by scanning electron micrographs, and the interaction of hemoglobin (Hb) with CMK-3 was studied by UV–vis spectroscopy and electrochemical methods. Under optimal conditions, {Hb/CMK-3}6film showed a couple of stable and well-defined redox peaks at about −377 and −296mV in pH 7.0 buffers. Furthermore, the {Hb/CMK-3}6film displayed excellent electrocatalysis to the reduction of both H2O2and O2. Based on thus-formed film and its direct electron transfer behavior, a novel biosensor was presented for the determination of H2O2ranging from 1.2 to 57μM with the detection limit of 0.6μM at S/N=3. CMK-3 provided a desirable matrix for protein immobilization and biosensor preparation.