Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure
Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure
复制标题
急性氨暴露后海洋青鳉 (Oryzias melastigma) 的蛋白质组学和代谢组学分析
DOI：
10.1007/s10646-017-1892-2
复制
发表时间：
2018-04-01
期刊：
影响因子：
2.7
通讯作者：
Zhang, Li
中科院分区：
文献类型：
--
作者：
Zhu, Limei;Gao, Na;Zhang, Li
氨是一种剧毒的环境污染物，也是嗜氨硬骨鱼产生的主要含氮废物。虽然氨对鱼类的急性毒性作用已被广泛研究，但其毒性的生化机制尚未全面了解。在这项研究中，我们对氨胁迫（1.2和2.6 mmol L−1NH4Cl处理96 h）和对照组的海洋medaka （Oryzias melastigma）进行了比较蛋白质组学和代谢组学分析，以确定氨胁迫下代谢物和蛋白质谱的变化。氨暴露后的代谢反应包括多种氨基酸、碳水化合物（葡萄糖和糖原）、能量代谢产物（ATP和肌酐）以及其他代谢产物（胆碱和磷胆碱）的变化，说明氨主要引起能量代谢和氨基酸代谢的紊乱。基于二维电泳的蛋白质组学研究鉴定了23个改变的蛋白，这些蛋白涉及神经系统、运动系统、细胞骨架组装、免疫应激、氧化应激和细胞凋亡的信号转导。上述结果表明，氨不仅会引起海洋medaka的氧化应激、免疫应激、细胞损伤和凋亡，还会影响运动能力和中枢神经系统。这是首次将代谢组学和蛋白质组学方法结合起来阐明海洋鱼类的氨毒性。本研究对深入了解海洋鱼类氨毒性机理和水产养殖实践具有重要价值。
Ammonia is both a highly toxic environmental pollutant and the major nitrogenous waste produced by ammoniotelic teleosts. Although the acute toxic effects of ammonia have been widely studied in fish, the biochemical mechanisms of its toxicity have not been understood comprehensively. In this study, we performed comparative proteomic and metabolomic analysis between ammonia-challenged (1.2 and 2.6 mmol L−1NH4Cl for 96 h) and control groups of marine medaka (Oryzias melastigma) to identify changes of the metabolite and protein profiles in response to ammonia stress. The metabolic responses included changes of multiple amino acids, carbohydrates (glucose and glycogen), energy metabolism products (ATP and creatinine), and other metabolites (choline and phosphocholine) after ammonia exposure, indicating that ammonia mainly caused disturbance in energy metabolism and amino acids metabolism. The two-dimensional electrophoresis-based proteomic study identified 23 altered proteins, which were involved in nervous system, locomotor system, cytoskeleton assembly, immune stress, oxidative stress, and signal transduction of apoptosis. These results suggested that ammonia not only induced oxidative stress, immune stress, cell injury and apoptosis but also affected the motor ability and central nervous system in marine medaka. It is the first time that metabolomic and proteomic approaches were integrated to elucidate ammonia toxicity in marine fishes. This study is of great value in better understanding the mechanisms of ammonia toxicity in marine fishes and in practical aspects of aquaculture.