Long-chain n-3 fatty acids enhance neonatal insulin-regulated protein metabolism in piglets by differentially altering muscle lipid composition
Long-chain n-3 fatty acids enhance neonatal insulin-regulated protein metabolism in piglets by differentially altering muscle lipid composition
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DOI：
10.1194/jlr.m700166-jlr200
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发表时间：
2007-11-01
影响因子：
6.5
通讯作者：
Thivierge, M. Carole
中科院分区：
文献类型：
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作者：
Bergeron, Karen;Julien, Pierre;Thivierge, M. Carole
本研究探讨了肌肉磷脂中长链n-3多不饱和脂肪酸（LCn-3 PUFAs）在新生儿代谢调节中的作用。28头仔猪在2日龄时断奶，并以两种配方奶中的一种喂养，所述配方奶由供应0%的对照配方奶或含有3.5%LCn-3 PUFA的配方奶组成，直至10或28日龄。在出生后的第一个月内，对照猪对氨基酸处理的胰岛素敏感性出现发育性下降，使用高胰岛素-正常血糖-正常氨基酸钳夹评估，每单位胰岛素增量的斜率为-2.24 μ mol中心点kg（-1）中心点h（-1）（P = 0.01）。LCn-3 PUFA喂养减弱了这种发育下降，导致不同的胰岛素敏感性（P < 0.001）。当在胃肠外喂养诱导的高胰岛素血症下评估蛋白质代谢时，LCn-3 PUFAs减少了16%的全身氨基酸氧化损失（从238到231 μ mol中心点kg（-1）中心点h（-1）; P = 0.06），允许41%以上的氨基酸附着到体内蛋白质中（从90到127 μ mol中心点kg（-1）中心点h（-1）; P = 0.06）。肌肉混合蛋白的合成率分数保持不变的LCn-3 PUFA喂养。然而，LCn-3 PUFAs延缓了肌肉细胞内游离池的必需与非必需氨基酸比率的发育增加（P = 0.05）。总体而言，代谢的改变伴随着LCn-3 PUFAs优先掺入肌肉总膜磷脂（P，0.001），与肌内甘油三酯相反。这些结果强调了LCn-3 PUFAs作为新生儿蛋白质代谢不同方面的调节剂的潜在作用。
This study investigated the role of long-chain n-3 polyunsaturated fatty acids ( LCn-3PUFAs) of muscle phospholipids in the regulation of neonatal metabolism. Twenty-eight piglets were weaned at 2 days of age and raised on one of two milk formulas that consisted of either a control formula supplying 0% or a formula containing 3.5% LCn-3PUFAs until 10 or 28 days of age. There was a developmental decline in the insulin sensitivity of amino acid disposal in control pigs during the first month of life, with a slope of -2.24 mu mol center dot kg(-1)center dot h(-1) (P = 0.01) per unit of insulin increment, as assessed using hyperinsulinemic-euglycemic-euaminoacidemic clamps. LCn-3PUFA feeding blunted this developmental decline, resulting in differing insulin sensitivities (P < 0.001). When protein metabolism was assessed under parenteral feeding-induced hyperinsulinemia, LCn-3PUFAs reduced by 16% whole body oxidative losses of amino acids (from 238 to 231 mu mol center dot kg(-1)center dot h(-1); P = 0.06), allowing 41% more amino acids to accrete into body proteins (from 90 to 127 mu mol center dot kg(-1)center dot h(-1); P = 0.06). The fractional synthetic rate of muscle mixed proteins remained unaltered by the LCn-3PUFA feeding. However, LCn-3PUFAs retarded a developmental increase in the essential-to-nonessential amino acid ratio of the muscle intracellular free pool (P = 0.05). Overall, alterations in metabolism were concomitant with a preferential incorporation of LCn-3PUFAs into muscle total membrane phospholipids ( P, 0.001), in contrast to intramuscular triglycerides. These results underscore the potential role of LCn-3PUFAs as regulators of different aspects of protein metabolism in the neonate.