Microarray profiling of gene expression in human adipocytes in response to anthocyanins
Microarray profiling of gene expression in human adipocytes in response to anthocyanins
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DOI：
10.1016/j.bcp.2005.12.042
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发表时间：
2006-04-14
影响因子：
5.8
通讯作者：
Osawa, T
中科院分区：
文献类型：
--
作者：
Tsuda, T;Ueno, Y;Osawa, T
脂肪细胞功能障碍与肥胖和胰岛素抵抗的发展密切相关。公认调节脂肪细胞因子分泌或脂肪细胞特异性基因表达是预防肥胖和改善胰岛素敏感性的最重要的靶点之一。近年来，我们发现花青素作为一种广泛存在于植物界的色素，具有抗小鼠肥胖、促进脂肪细胞分泌脂肪细胞因子及其基因表达的作用。在这项研究中，我们已经显示了基因表达谱在人类脂肪细胞处理花青素（花青素3-葡萄糖苷; C3 G或花青素; Cy）。用100 μ M C3 G、Cy或赋形剂处理人脂肪细胞24小时。从脂肪细胞中提取总RNA并进行基因芯片分析。基于基因表达谱，我们证明了脂肪细胞因子表达的显著变化（脂联素上调，纤溶酶原激活物抑制剂-1和白细胞介素-6下调）。C3 G和Cy处理组的解偶联蛋白2、脂酰辅酶A氧化酶1和围脂蛋白表达均显著增加。这些研究提供了花青素处理的人脂肪细胞基因表达谱的概述，并证明花青素可以调节脂肪细胞因子基因表达，以改善与肥胖和糖尿病相关的脂肪细胞功能，值得进一步研究。(c)2006年爱思唯尔公司All rights reserved.
Adipocyte dysfunction is strongly associated with the development of obesity and insulin resistance. it is accepted that the regulation of adipocytokine secretion or the adipocyte specific gene expression is one of the most important targets for the prevention of obesity and amelioration of insulin sensitivity. Recently, we demonstrated that anthocyanins, which are pigments widespread in the plant kingdom, have the potency of anti-obesity in mice and the enhancement adipocytokine secretion and its gene expression in adipocytes. In this study, we have shown the gene expression profile in human adipocytes treated with anthocyanins (cyanidin 3-glucoside; C3G or cyanidin; Cy). The human adipocytes were treated with 100 mu M C3G, Cy or vehicle for 24 h. The total RNA from the adipocytes was isolated and carried out GeneChip microarray analysis. Based on the gene expression profile, we demonstrated the significant changes of adipocytokine expression (up-regulation of adiponectin and down-regulation of plasminogen activator inhibitor-1 and interieukin-6). Some of lipid metabolism related genes (uncoupling protein2, acylCoA oxidase1 and perilipin) also significantly induced in both common the C3G or Cy treatment groups. These studies have provided an overview of the gene expression profiles in human adipocytes treated with anthocyanins and demonstrated that anthocyanins can regulate adipocytokine gene expression to ameliorate adipocyte function related with obesity and diabetes that merit further investigation. (c) 2006 Elsevier Inc. All rights reserved.