Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin
Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin
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DOI：
10.2166/wst.1995.0287
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发表时间：
1995-01-01
影响因子：
2.7
通讯作者：
Nielsen, PH
中科院分区：
文献类型：
--
作者：
Jahn, A;Nielsen, PH
采用阳离子交换树脂从不同的污水生物膜和恶臭杆菌生物膜单培养中提取水溶性EPS材料。这种提取方法似乎特别适合从生物膜中提取腐殖质物质和蛋白质，但根据样品的性质，也可以提取碳水化合物。萃取条件受搅拌强度、阳离子交换树脂用量和萃取时间的影响，必须明确萃取条件。在dowex可提取的部分中发现了高达20%的总生物膜蛋白。吖啶橙（AO）直接计数在提取时间2小时内未检测到明显的细胞裂解。将提取方法应用于恶臭杆菌的批量培养时，结果也相同。然而，在提取30分钟后，通过平板计数技术记录的批量培养中活细胞的数量减少到28%。综上所述，从生物膜EPS中可以提取出大量的蛋白质和腐殖质物质，在分析生物膜EPS基质的特性时应进行更详细的研究。
A cation exchange resin was used to extract water soluble EPS material from different sewer biofilms and a biofilm monoculture of P. putida. The extraction method seemed to be especially suitable for extracting humic substances and proteins from biofilms but also carbohydrate could be extracted, depending on the nature of the sample. Extraction conditions must be well defined because the extraction efficiency was dependent on stirring intensity, amount of cation exchange resin and extraction time. Up to 20% of the total biofilm protein was found in the DOWEX-extractable fraction. No significant cell-lysis up to 2 hours of extraction time could be detected by acridine orange (AO) direct counts. The same was true when applying the extraction method to a batch culture of P. putida. However, the number of viable cells in the batch culture as recorded by plate count technique was reduced to 28% after 30 min of extraction. In conclusion, proteins and humic substances can be extracted from biofilm EPS in considerable amounts and should be investigated more in detail when analysing the properties of the EPS matrix of a biofilm.