Dynamic changes in expression of heme oxygenases in mouse heart and liver during hypoxia
Dynamic changes in expression of heme oxygenases in mouse heart and liver during hypoxia
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DOI：
10.1016/j.bbrc.2005.08.100
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发表时间：
2005-12-09
影响因子：
3.1
通讯作者：
Shibahara, S
中科院分区：
文献类型：
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作者：
Han, F;Takeda, K;Shibahara, S
血红素加氧酶分解血红素形成胆绿素、一氧化碳(CO)和铁，由两个结构上相关的同工酶HO-1和HO-2组成。HO-2也被认为是一种潜在的氧气传感器。反映内源性血红素降解总量的动脉血中CO相对含量在小鼠适应常压低氧(10%O-2)的过程中发生动态变化，在低氧第1天和第21天出现两个峰值。缺氧7d小鼠肝脏HO-1和HO-2蛋白表达水平分别下降20%和40%，14d时恢复到基础水平。另一方面，在低氧28天，心脏中的HO-1和HO-2蛋白分别增加了2倍和1.3倍。因此，低氧在体内诱导或抑制HO-1和HO-2的表达取决于细胞微环境。(C)2005 Elsevier Inc.保留所有权利。
Heme oxygenase cleaves heme to form biliverdin, carbon monoxide (CO), and iron, and consists of two structurally related isozymes, HO-1 and HO-2. HO-2 is also known as a potential oxygen sensor. Here we show that the relative CO content in arterial blood, which reflects the total amount of endogenous heme degradation, dynamically changes in mice during acclimatization to normobaric hypoxia (10% O-2), with the two peaks at 1 day and 21 days of hypoxia. The expression levels of HO-1 and HO-2 proteins were decreased by 20% and 40%, respectively in the mouse liver at 7 days of hypoxia, which returned to the basal levels at 14 days. On the other hand, HO-1 and HO-2 proteins were increased 2-fold and 1.3-fold, respectively, in the heart at 28 days of hypoxia. Thus, hypoxia induces or represses the expression of HO-1 and HO-2 in vivo, depending on cellular microenvironments. (c) 2005 Elsevier Inc. All rights reserved.